Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle
- Authors
- Kim, Narae; Jang, Yunho; Kim, Jin Kyoung; Ryoo, Soyoon; Kwon, Ka Hee; Kim, Miso; Kang, Shin Seok; Byeon, Hyeon Seop; Lee, Hee Soo; Lim, Young-Hee; Kim, Jae-Myung
- Issue Date
- 2017
- Publisher
- KOREAN SOC VETERINARY SCIENCE
- Keywords
- Korea; Mycobacterium bovis; cattle; genomics
- Citation
- JOURNAL OF VETERINARY SCIENCE, v.18, pp.333 - 341
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF VETERINARY SCIENCE
- Volume
- 18
- Start Page
- 333
- End Page
- 341
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/86431
- DOI
- 10.4142/jvs.2017.18.S1.333
- ISSN
- 1229-845X
- Abstract
- The aim of this study was to investigate the molecular characteristics and to conduct a comparative genomic analysis of Mycobacterium (M.) bovis strain 1595 isolated from a native Korean cow. Molecular typing showed that M. bovis 1595 has spoligotype SB0140 with mycobacterial interspersed repetitive units-variable number of tandem repeats typing of 4-2-5-3-2-7-5-5-4-3-4-3-4-3, representing the most common type of M. bovis in Korea. The complete genome sequence of strain 1595 was determined by single-molecule real-time technology, which showed a genome of 4351712 bp in size with a 65.64% G + C content and 4358 protein-coding genes. Comparative genomic analysis with the genomes of Mycobacterium tuberculosis complex strains revealed that all genomes are similar in size and G + C content. Phylogenetic analysis revealed all strains were within a 0.1% average nucleotide identity value, and MUMmer analysis illustrated that all genomes showed positive collinearity with strain 1595. A sequence comparison based on BLASTP analysis showed that M. bovis AF2122/97 was the strain with the greatest number of completely matched proteins to M. bovis 1595. This genome sequence analysis will serve as a valuable reference for improving understanding of the virulence and epidemiologic traits among M. bovis isolates in Korea.
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