Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

In vitro and in vivo comparison of the immunotoxicity of single- and multi-layered graphene oxides with or without pluronic F-127

Authors
Cho, Young CholPak, Pyo JuneJoo, Yong HoonLee, Hoi-SeonChung, Namhyun
Issue Date
12-12월-2016
Publisher
NATURE PUBLISHING GROUP
Citation
SCIENTIFIC REPORTS, v.6
Indexed
SCIE
SCOPUS
Journal Title
SCIENTIFIC REPORTS
Volume
6
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/86551
DOI
10.1038/srep38884
ISSN
2045-2322
Abstract
Graphene oxide (GO) has been a focus of research in the fields of electronics, energy, and biomedicine, including drug delivery. Thus, single- and multi-layered GO (SLGO and MLGO) have been produced and investigated. However, little information on their toxicity and biocompatibility is available. In the present study, we performed a comprehensive study of the size-and dose-dependent toxicity of GOs in the presence or absence of Pluronic F-127 on THP-1 cells by examining their viability, membrane integrity, levels of cytokine and ROS production, phagocytosis, and cytometric apoptosis. Moreover, as an extended study, a toxicity evaluation in the acute and chronic phases was performed in mice via intravenous injection of the materials. GOs exhibited dose-and size-dependent toxicity. Interestingly, SLGO induced ROS production to a lesser extent than MLGO. Cytometric analysis indicated that SLGO induced necrosis and apoptosis to a lesser degree than MLGO. In addition, cell damage and IL-1 beta production were influenced by phagocytosis. A histological animal study revealed that GOs of various sizes induced acute and chronic damage to the lung and kidney in the presence or absence of Pluronic F-127. These results will facilitate studies of GO prior to its biomedical application.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biosystems and Biotechnology > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Chung, Nam hyun photo

Chung, Nam hyun
융합생명공학과
Read more

Altmetrics

Total Views & Downloads

BROWSE