Enhanced production of 2,3-butanediol by engineered Saccharomyces cerevisiae through fine-tuning of pyruvate decarboxylase and NADH oxidase activities
- Authors
- Kim, Jin-Woo; Kim, Jungyeon; Seo, Seung-Oh; Kim, Kyoung Heon; Jin, Yong-Su; Seo, Jin-Ho
- Issue Date
- 9-Dec-2016
- Publisher
- BMC
- Keywords
- Pyruvate decarboxylase; Saccharomyces cerevisiae; 2, 3-Butanediol; NADH oxidase; Metabolomics; Metabolic engineering
- Citation
- BIOTECHNOLOGY FOR BIOFUELS, v.9
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOTECHNOLOGY FOR BIOFUELS
- Volume
- 9
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/86555
- DOI
- 10.1186/s13068-016-0677-9
- ISSN
- 1754-6834
- Abstract
- Background: 2,3-Butanediol (2,3-BD) is a promising compound for various applications in chemical, cosmetic, and agricultural industries. Pyruvate decarboxylase (Pdc)-deficient Saccharomyces cerevisiae is an attractive host strain for producing 2,3-BD because a large amount of pyruvate could be shunted to 2,3-BD production instead of ethanol synthesis. However, 2,3-BD yield, productivity, and titer by engineered yeast were inferior to native bacterial producers because of the following metabolic limitations. First, the Pdc-deficient yeast showed growth defect due to a shortage of C-2-compounds. Second, redox imbalance during the 2,3-BD production led to glycerol formation that lowered the yield. Results: To overcome these problems, the expression levels of Pdc from a Crabtree-negative yeast were optimized in S. cerevisiae. Specifically, Candida tropicalis PDC1 (CtPDC1) was used to minimize the production of ethanol but maximize cell growth and 2,3-BD productivity. As a result, productivity of the BD5_G1CtPDC1 strain expressing an optimal level of Pdc was 2.3 folds higher than that of the control strain in flask cultivation. Through a fed-batch fermentation, 121.8 g/L 2,3-BD was produced in 80 h. NADH oxidase from Lactococcus lactis (noxE) was additionally expressed in the engineered yeast with an optimal activity of Pdc. The fed-batch fermentation with the optimized 2-stage aeration control led to production of 154.3 g/L 2,3-BD in 78 h. The overall yield of 2,3-BD was 0.404 g 2,3-BD/g glucose which corresponds to 80.7% of theoretical yield. Conclusions: A massive metabolic shift in the engineered S. cerevisiae (BD5_G1CtPDC1_nox) expressing NADH oxidase was observed, suggesting that redox imbalance was a major bottleneck for efficient production of 2,3-BD by engineered yeast. Maximum 2,3-BD titer in this study was close to the highest among the reported microbial production studies. The results demonstrate that resolving both C-2-compound limitation and redox imbalance is critical to increase 2,3-BD production in the Pdc-deficient S. cerevisiae. Our strategy to express fine-tuned PDC and noxE could be applicable not only to 2,3-BD production, but also other chemical production systems using Pdc-deficient S. cerevisiae.
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