Protective Effect of Deer Bone Oil on Cartilage Destruction in Rats with Monosodium Iodoacetate (MIA)-Induced Osteoarthritis
DC Field | Value | Language |
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dc.contributor.author | Choi, Hyeon-Son | - |
dc.contributor.author | Im, Suji | - |
dc.contributor.author | Park, Je Won | - |
dc.contributor.author | Suh, Hyung Joo | - |
dc.date.accessioned | 2021-09-03T16:02:43Z | - |
dc.date.available | 2021-09-03T16:02:43Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2016-12 | - |
dc.identifier.issn | 0918-6158 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/86593 | - |
dc.description.abstract | The anti-osteoarthritic activity of the methanol fraction of deer bone oil extract (DBO-M) was evaluated in interleukin (IL)-1 beta-inflamed primary rabbit chondrocytes and in rats with monosodium iodoacetate (MIA)-induced osteoarthritis. The active compound in DBO-M was analyzed using a direct infusion liquid chromatography quadrupole (LCQ) ion-trap electrospray ionization (ESI)-mass spectrometer (MS). DBO-M significantly suppressed the IL-1 beta-induced sulfated-glycosaminoglycan (s-GAG) release from chondrocyte, and lowered mRNA levels of the collagen-degrading enzymes matrix metalloproteinase (MMP)-1 and MMP-3 in a dose-dependent manner. Upon treatment with high doses of DBO-M, the levels of IL-1 beta, tumor necrosis factor (TNF)-alpha, and IL-6 decreased by around 40, 70, and 50%, respectively, compared to the control in the serum of rats with MIA-induced osteoarthritis. Bone volume fraction (BV/TV) and trabecular thickness (Tb.Th) increased by over 40% in rats treated with DBO-M compared to the values reported for the MIA-treated control group, while trabecular separation (Tb.Sp) showed a significant decrease (ca. 38%), as confirmed through micro-computed tomography (CT) analysis of MIA-induced destruction of articular bones. Furthermore, direct infusion ESI-MS analysis showed that DBO-M contains gangliosides, which are glycosphingolipids with monosialic acid (GM3), as a major compound. Our results suggest that DBO-M effectively improves MIA-induced osteoarthritis by suppressing inflammatory responses, and that gangliosides could be one of the DSO-derived anti-inflammatory components. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PHARMACEUTICAL SOC JAPAN | - |
dc.subject | MATRIX METALLOPROTEINASES | - |
dc.subject | INFLAMMATORY RESPONSES | - |
dc.subject | CHONDROCYTE APOPTOSIS | - |
dc.subject | GANGLIOSIDES | - |
dc.subject | EXTRACT | - |
dc.subject | ANTLER | - |
dc.subject | CYTOKINES | - |
dc.subject | GLUTAMATE | - |
dc.subject | PROTEIN | - |
dc.subject | WEIGHT | - |
dc.title | Protective Effect of Deer Bone Oil on Cartilage Destruction in Rats with Monosodium Iodoacetate (MIA)-Induced Osteoarthritis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Je Won | - |
dc.contributor.affiliatedAuthor | Suh, Hyung Joo | - |
dc.identifier.doi | 10.1248/bpb.b16-00565 | - |
dc.identifier.scopusid | 2-s2.0-85006102572 | - |
dc.identifier.wosid | 000389014700018 | - |
dc.identifier.bibliographicCitation | BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.39, no.12, pp.2042 - 2051 | - |
dc.relation.isPartOf | BIOLOGICAL & PHARMACEUTICAL BULLETIN | - |
dc.citation.title | BIOLOGICAL & PHARMACEUTICAL BULLETIN | - |
dc.citation.volume | 39 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 2042 | - |
dc.citation.endPage | 2051 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | MATRIX METALLOPROTEINASES | - |
dc.subject.keywordPlus | INFLAMMATORY RESPONSES | - |
dc.subject.keywordPlus | CHONDROCYTE APOPTOSIS | - |
dc.subject.keywordPlus | GANGLIOSIDES | - |
dc.subject.keywordPlus | EXTRACT | - |
dc.subject.keywordPlus | ANTLER | - |
dc.subject.keywordPlus | CYTOKINES | - |
dc.subject.keywordPlus | GLUTAMATE | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | WEIGHT | - |
dc.subject.keywordAuthor | osteoarthritis (OA) | - |
dc.subject.keywordAuthor | deer bone oil | - |
dc.subject.keywordAuthor | chondrocyte | - |
dc.subject.keywordAuthor | monosodium iodoacetate (MIA) | - |
dc.subject.keywordAuthor | micro-computed tomography (Micro-CT) | - |
dc.subject.keywordAuthor | ganglioside | - |
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