The suppressive effects of metformin on inflammatory response of otitis media model in human middle ear epithelial cells
DC Field | Value | Language |
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dc.contributor.author | Cho, Jae Gu | - |
dc.contributor.author | Song, Jae Jun | - |
dc.contributor.author | Choi, June | - |
dc.contributor.author | Im, Gi Jung | - |
dc.contributor.author | Jung, Hak Hyun | - |
dc.contributor.author | Chae, Sung Won | - |
dc.date.accessioned | 2021-09-03T19:02:19Z | - |
dc.date.available | 2021-09-03T19:02:19Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2016-10 | - |
dc.identifier.issn | 0165-5876 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/87252 | - |
dc.description.abstract | Objective: Metformin is a well-known anti-diabetic agent, but its mechanism is unclear. Recently, many reports have described the anti-inflammatory effects of metformin on various cell types, including human vascular smooth muscle cells and endothelial cells. This study was designed to investigate the anti-inflammatory effect of metformin on lipopolysaccharide (LPS) induced inflammation in human middle ear epithelial cell lines (HMEECs). Methods: The effect of pretreatment by metformin (0, 1, 2, 4 mM) was evaluated by the inflammatory response in the HMEECs exposed to LPS (10 ng/m1). For verifying the suppression effect of metformin on the inflammatory cytokines, tumor necrosis factor-alpha (TNF-alpha) was evaluated by real-time polymerase chain reaction, and COX-2 protein was assessed by western blotting. Intracellular reactive oxygen species (ROS) was measured using 2', 7'-dichlorofluorescein diacetate (DCFHDA) fluorocytometer. Results: Stimulation by LPS 10 ng/ml concentration showed 12.4 folds increase the expression of TNF-alpha mRNA compared to control on HMEECs. Pretreatment of metformin dose dependently suppressed the expression of TNF-alpha mRNA induced by LPS (2 mM, p = 0.03). The amount of COX-2 protein production was significantly decreased by metformin pretreatment (4 mM, p = 0.01). The production of ROS was decreased significantly by pretreatment of metformin (p = 0.03). Conclusions: These findings suggest that the inflammatory response and oxidative stress induced by LPS could be suppressed by metformin in HMEECs. Therefore, metformin may have a therapeutic potential for the treatment of the otitis media. (C) 2016 Elsevier Ireland Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.subject | ACTIVATED PROTEIN-KINASE | - |
dc.subject | TUMOR-NECROSIS-FACTOR | - |
dc.subject | EFFUSIONS | - |
dc.subject | ENDOTOXIN | - |
dc.subject | INSULIN | - |
dc.title | The suppressive effects of metformin on inflammatory response of otitis media model in human middle ear epithelial cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Cho, Jae Gu | - |
dc.contributor.affiliatedAuthor | Song, Jae Jun | - |
dc.contributor.affiliatedAuthor | Choi, June | - |
dc.contributor.affiliatedAuthor | Im, Gi Jung | - |
dc.contributor.affiliatedAuthor | Jung, Hak Hyun | - |
dc.contributor.affiliatedAuthor | Chae, Sung Won | - |
dc.identifier.doi | 10.1016/j.ijporl.2016.07.025 | - |
dc.identifier.scopusid | 2-s2.0-84979639088 | - |
dc.identifier.wosid | 000383933500006 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY, v.89, pp.28 - 32 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY | - |
dc.citation.title | INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY | - |
dc.citation.volume | 89 | - |
dc.citation.startPage | 28 | - |
dc.citation.endPage | 32 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Otorhinolaryngology | - |
dc.relation.journalResearchArea | Pediatrics | - |
dc.relation.journalWebOfScienceCategory | Otorhinolaryngology | - |
dc.relation.journalWebOfScienceCategory | Pediatrics | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | TUMOR-NECROSIS-FACTOR | - |
dc.subject.keywordPlus | EFFUSIONS | - |
dc.subject.keywordPlus | ENDOTOXIN | - |
dc.subject.keywordPlus | INSULIN | - |
dc.subject.keywordAuthor | Metformin | - |
dc.subject.keywordAuthor | Otitis media | - |
dc.subject.keywordAuthor | LPS | - |
dc.subject.keywordAuthor | Human middle ear epitheilial cells | - |
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