Localized electroporation effect on adherent cells in modified electric cell-substrate impedance sensing circuits
- Authors
- Kim, Yu Jin; Song, Ka Ram; Kim, Hee-Dae; Park, Bum Chul; Kim, Young Keun; Kang, Chi Jung
- Issue Date
- 10월-2016
- Publisher
- IOP PUBLISHING LTD
- Citation
- APPLIED PHYSICS EXPRESS, v.9, no.10
- Indexed
- SCIE
SCOPUS
- Journal Title
- APPLIED PHYSICS EXPRESS
- Volume
- 9
- Number
- 10
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/87263
- DOI
- 10.7567/APEX.9.107001
- ISSN
- 1882-0778
- Abstract
- Electroporation is a physical transfection method for introducing foreign genes or drugs into cells. It does not require toxic reagents or transfection vectors. However, its applications have been limited because of cell damage and nonspecific transport. Here, we present an effective method for selective and localized electroporation using atomic force microscopy. This electroporation method is applied to adherent cells on substrates, instead of conventionally used suspended cells, and offers relatively effective cell transfection. Moreover, this method enables localized transfection into targeted areas at the single-cell level. (C) 2016 The Japan Society of Applied Physics
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Engineering > Department of Materials Science and Engineering > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.