Analysis of single nucleotide polymorphism among Varicella-Zoster Virus and identification of vaccine-specific sites
- Authors
- Jeon, Jeong Seon; Won, Youn Hee; Kim, In Kyo; Ahn, Jin Hyun; Shin, Ok Sarah; Kim, Jung Hwan; Lee, Chan Hee
- Issue Date
- 9월-2016
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Varicella-zoster virus (VZV); Live attenuated vaccine; Single nucleotide polymorphism (SNP); Vaccine-specific sites; Sequence diversity; Genetic heterogeneity
- Citation
- VIROLOGY, v.496, pp.277 - 286
- Indexed
- SCIE
SCOPUS
- Journal Title
- VIROLOGY
- Volume
- 496
- Start Page
- 277
- End Page
- 286
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/87559
- DOI
- 10.1016/j.virol.2016.06.017
- ISSN
- 0042-6822
- Abstract
- Varicella-zoster virus (VZV) is a causative agent for chickenpox and zoster. Live attenuated vaccines have been developed based on Oka and MAV/06 strains. In order to understand the molecular mechanisms of attenuation, complete genome sequences of vaccine and wild-type strains were compared and single nucleotide polymorphism (SNP) was analyzed. ORF22 and ORF62 contained the highest number of SNPs. The detailed analysis of the SNPs suggested 24 potential vaccine-specific sites. All the mutational events found in vaccine-specific sites were transitional, and most of them were substitution of AT to GC pair. Interestingly, 18 of the vaccine-specific sites of the vaccine strains appeared to be genetically heterogeneous. The probability of a single genome of vaccine strain to contain all 24 vaccine-type sequences was calculated to be less than 4%. The average codon adaptation index (CAI) value of the vaccine strains was significantly lower than the CAI value of the clinical strains. (C) 2016 Elsevier Inc. All rights reserved.
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