Amelioration of an LPS-induced inflammatory response using a methanolic extract of Lagerstroemia ovalifolia to suppress the activation of NF-B in RAW264.7 macrophages
- Authors
- Park, Ji-Won; Kwon, Ok-Kyoung; Yuniato, Prasetyawan; Marwoto, Bambang; Lee, Joongku; Oh, Sei-Ryang; Kim, Jae-Hong; Ahn, Kyung-Seop
- Issue Date
- 8월-2016
- Publisher
- SPANDIDOS PUBL LTD
- Keywords
- Lagerstroemia ovalifolia Teijsm; & Binn; inflammation; lipopolysaccharide; MARK; NF-B
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, v.38, no.2, pp.482 - 490
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
- Volume
- 38
- Number
- 2
- Start Page
- 482
- End Page
- 490
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/87918
- DOI
- 10.3892/ijmm.2016.2646
- ISSN
- 1107-3756
- Abstract
- Lagerstroemia ovalifolia Teijsm. & Binn. has traditionally been used as an herbal medicine and possesses anti-inflammatory properties. However, the mechanisms underlying its anti-inflammatory effects remain poorly understood. For this purpose, we aimed to investigate the effects of methanolic extract of L. ovalifolia (LOME) on nitric oxide (NO) and prostaglandin E-2 (PGE(2)) production, as well as the underlying molecular mechanisms responsible for these effects, in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. We examined the effects of LOME on the production of NO and PGE(2) in LPS-stimulated RAW264.7 cells. To explore the anti-inflammatory mechanisms of LOME, we measured the mRNA or protein expression of the pro-inflammatory mediators induced by LOME in the LPS-stimulated RAW264.7 cells. LOME significantly inhibited the production of NO, PGE(2), interleukin (IL)-6, IL-1, and tumor necrosis factor- (TNF-) in LPS-stimulated RAW264.7 cells. Moreover, LOME suppressed the mRNA and protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and inhibited the phosphorylation of the mitogen-activated protein kinases (MAPKs), with a reduction in the nuclear translocation of nuclear factor (NF)-B in LPS-stimulated RAW264.7 cells. Taken together, these findings suggest that LOME may exert anti-inflammatory effects in vitro in LPS-stimulated RAW264.7 macrophages and thus, may have potential for use as an adjuvant treatment of inflammatory diseases.
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Collections - College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
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