Anti-inflammatory effects of Viola yedoensis and the application of cell extraction methods for investigating bioactive constituents in macrophages
DC Field | Value | Language |
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dc.contributor.author | Jeong, Yun Hee | - |
dc.contributor.author | Oh, You-Chang | - |
dc.contributor.author | Cho, Won-Kyung | - |
dc.contributor.author | Shin, Hyeji | - |
dc.contributor.author | Lee, Ki Yong | - |
dc.contributor.author | Ma, Jin Yeul | - |
dc.date.accessioned | 2021-09-03T22:53:18Z | - |
dc.date.available | 2021-09-03T22:53:18Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2016-06-14 | - |
dc.identifier.issn | 1472-6882 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/88338 | - |
dc.description.abstract | Background: Viola yedoensis (VY, Violaceae) is a popular medicinal herb used in traditional eastern medicine for treating lots of diseases, including inflammation and its related symptoms. However, the anti-inflammatory properties of VY have not been demonstrated. In the present study, we investigated the anti-inflammatory effects of VY ethanol extract (VYE) on macrophages and attempted to identify the bioactive components of VYE. Methods: We assessed the effects of VYE on secretion of nitric oxide (NO) and inflammatory cytokines such as tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-1 beta. In addition, we explored the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, and changes in heme oxygenase (HO)-1, nuclear factor (NF)-kappa B, and mitogen-activated protein kinase (MAPK) signaling pathways in RAW 264.7 macrophages stimulated by lipopolysaccharide (LPS). In addition, a rapid and useful approach to identify potential bioactive components in VYE with anti-inflammatory effects was developed using murine macrophage cell extraction coupled with high-performance liquid chromatography tandem mass spectrometry (LC-MS). Results: We found that VYE exerted anti-inflammatory activity by inhibiting the production of key inflammation mediators and related products, as well as suppression of HO-1, NF-kappa B, and MAPK signaling pathway activation in RAW 264.7 cells. In addition, we identified two compounds in VYE via the cell extraction method. Conclusions: Our results revealed that VYE exerts anti-inflammatory activities and its detailed inhibitory mechanism in macrophages. Furthermore, we identified bioactive components of VYE. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | NITRIC-OXIDE SYNTHASE | - |
dc.subject | RAW 264.7 MACROPHAGES | - |
dc.subject | C-GLYCOSIDES | - |
dc.subject | ENDOTHELIAL-CELLS | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | IN-VITRO | - |
dc.subject | LIPOPOLYSACCHARIDE | - |
dc.subject | INFLAMMATION | - |
dc.subject | SUPPRESSION | - |
dc.title | Anti-inflammatory effects of Viola yedoensis and the application of cell extraction methods for investigating bioactive constituents in macrophages | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Ki Yong | - |
dc.identifier.doi | 10.1186/s12906-016-1142-9 | - |
dc.identifier.scopusid | 2-s2.0-84974815601 | - |
dc.identifier.wosid | 000377780900001 | - |
dc.identifier.bibliographicCitation | BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, v.16 | - |
dc.relation.isPartOf | BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE | - |
dc.citation.title | BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE | - |
dc.citation.volume | 16 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Integrative & Complementary Medicine | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | NITRIC-OXIDE SYNTHASE | - |
dc.subject.keywordPlus | RAW 264.7 MACROPHAGES | - |
dc.subject.keywordPlus | C-GLYCOSIDES | - |
dc.subject.keywordPlus | ENDOTHELIAL-CELLS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | LIPOPOLYSACCHARIDE | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | SUPPRESSION | - |
dc.subject.keywordAuthor | Viola yedoensis ethanol extract | - |
dc.subject.keywordAuthor | Nuclear factor-kappaB | - |
dc.subject.keywordAuthor | Mitogen-activated protein kinase | - |
dc.subject.keywordAuthor | Heme oxygenase-1 | - |
dc.subject.keywordAuthor | Cell extraction | - |
dc.subject.keywordAuthor | Bio-active components | - |
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