Anti-inflammatory effects of Viola yedoensis and the application of cell extraction methods for investigating bioactive constituents in macrophages
- Authors
- Jeong, Yun Hee; Oh, You-Chang; Cho, Won-Kyung; Shin, Hyeji; Lee, Ki Yong; Ma, Jin Yeul
- Issue Date
- 14-6월-2016
- Publisher
- BIOMED CENTRAL LTD
- Keywords
- Viola yedoensis ethanol extract; Nuclear factor-kappaB; Mitogen-activated protein kinase; Heme oxygenase-1; Cell extraction; Bio-active components
- Citation
- BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, v.16
- Indexed
- SCIE
SCOPUS
- Journal Title
- BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
- Volume
- 16
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/88338
- DOI
- 10.1186/s12906-016-1142-9
- ISSN
- 1472-6882
- Abstract
- Background: Viola yedoensis (VY, Violaceae) is a popular medicinal herb used in traditional eastern medicine for treating lots of diseases, including inflammation and its related symptoms. However, the anti-inflammatory properties of VY have not been demonstrated. In the present study, we investigated the anti-inflammatory effects of VY ethanol extract (VYE) on macrophages and attempted to identify the bioactive components of VYE. Methods: We assessed the effects of VYE on secretion of nitric oxide (NO) and inflammatory cytokines such as tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-1 beta. In addition, we explored the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, and changes in heme oxygenase (HO)-1, nuclear factor (NF)-kappa B, and mitogen-activated protein kinase (MAPK) signaling pathways in RAW 264.7 macrophages stimulated by lipopolysaccharide (LPS). In addition, a rapid and useful approach to identify potential bioactive components in VYE with anti-inflammatory effects was developed using murine macrophage cell extraction coupled with high-performance liquid chromatography tandem mass spectrometry (LC-MS). Results: We found that VYE exerted anti-inflammatory activity by inhibiting the production of key inflammation mediators and related products, as well as suppression of HO-1, NF-kappa B, and MAPK signaling pathway activation in RAW 264.7 cells. In addition, we identified two compounds in VYE via the cell extraction method. Conclusions: Our results revealed that VYE exerts anti-inflammatory activities and its detailed inhibitory mechanism in macrophages. Furthermore, we identified bioactive components of VYE.
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Collections - College of Pharmacy > Department of Pharmaceutical Science > 1. Journal Articles
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