Synaptic compartmentalization by micropatterned masking of a surface adhesive cue in cultured neurons
DC Field | Value | Language |
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dc.contributor.author | Ryu, Jae Ryun | - |
dc.contributor.author | Jang, Min Jee | - |
dc.contributor.author | Jo, Youhwa | - |
dc.contributor.author | Joo, Sunghoon | - |
dc.contributor.author | Lee, Do Hoon | - |
dc.contributor.author | Lee, Byung Yang | - |
dc.contributor.author | Nam, Yoonkey | - |
dc.contributor.author | Sun, Woong | - |
dc.date.accessioned | 2021-09-03T23:10:21Z | - |
dc.date.available | 2021-09-03T23:10:21Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2016-06 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/88406 | - |
dc.description.abstract | Functions of neuronal circuit are fundamentally modulated by its quality and quantity of connections. Assessment of synapse, the basic unit for a neuronal connection, is labor-intensive and time-consuming in conventional culture systems, due to the small size and the spatially random distribution. In the present study, we propose a novel 'synapse compartmentalization' culture system, in which synapses are concentrated at controlled locations. We fabricated a negative dot array pattern by coating the entire surface with poly-l-lysine (PLL) and subsequent microcontact printing of 1) substrates which mask positive charge of NI (Fc, BSA and laminin), or 2) a chemorepulsive protein (Semaphorin 3F-Fc). By combination of physical and biological features of these repulsive substrates, functional synapses were robustly concentrated in the PLL-coated dots. This synapse compartmentalization chip can be combined with the various high-throughput assay formats based on the synaptic morphology and function. Therefore, this quantifiable and controllable dot array pattern by microcontact printing will be potential useful for bio-chip platforms for the high-density assays used in synapse-related neurobiological studies. (C) 2016 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | GUIDANCE | - |
dc.subject | GROWTH | - |
dc.subject | SPECIFICATION | - |
dc.subject | MATURATION | - |
dc.subject | MOLECULES | - |
dc.subject | MIGRATION | - |
dc.subject | PROTEIN | - |
dc.subject | RHO | - |
dc.title | Synaptic compartmentalization by micropatterned masking of a surface adhesive cue in cultured neurons | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Byung Yang | - |
dc.contributor.affiliatedAuthor | Sun, Woong | - |
dc.identifier.doi | 10.1016/j.biomaterials.2016.03.027 | - |
dc.identifier.scopusid | 2-s2.0-84973534651 | - |
dc.identifier.wosid | 000375366200005 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.92, pp.46 - 56 | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 92 | - |
dc.citation.startPage | 46 | - |
dc.citation.endPage | 56 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | GUIDANCE | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | SPECIFICATION | - |
dc.subject.keywordPlus | MATURATION | - |
dc.subject.keywordPlus | MOLECULES | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | RHO | - |
dc.subject.keywordAuthor | Microcontact printing | - |
dc.subject.keywordAuthor | Synapse compartmentalization | - |
dc.subject.keywordAuthor | Bioassay chip | - |
dc.subject.keywordAuthor | Neuronal culture | - |
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