Synaptic compartmentalization by micropatterned masking of a surface adhesive cue in cultured neurons
- Authors
- Ryu, Jae Ryun; Jang, Min Jee; Jo, Youhwa; Joo, Sunghoon; Lee, Do Hoon; Lee, Byung Yang; Nam, Yoonkey; Sun, Woong
- Issue Date
- 6월-2016
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Microcontact printing; Synapse compartmentalization; Bioassay chip; Neuronal culture
- Citation
- BIOMATERIALS, v.92, pp.46 - 56
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOMATERIALS
- Volume
- 92
- Start Page
- 46
- End Page
- 56
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/88406
- DOI
- 10.1016/j.biomaterials.2016.03.027
- ISSN
- 0142-9612
- Abstract
- Functions of neuronal circuit are fundamentally modulated by its quality and quantity of connections. Assessment of synapse, the basic unit for a neuronal connection, is labor-intensive and time-consuming in conventional culture systems, due to the small size and the spatially random distribution. In the present study, we propose a novel 'synapse compartmentalization' culture system, in which synapses are concentrated at controlled locations. We fabricated a negative dot array pattern by coating the entire surface with poly-l-lysine (PLL) and subsequent microcontact printing of 1) substrates which mask positive charge of NI (Fc, BSA and laminin), or 2) a chemorepulsive protein (Semaphorin 3F-Fc). By combination of physical and biological features of these repulsive substrates, functional synapses were robustly concentrated in the PLL-coated dots. This synapse compartmentalization chip can be combined with the various high-throughput assay formats based on the synaptic morphology and function. Therefore, this quantifiable and controllable dot array pattern by microcontact printing will be potential useful for bio-chip platforms for the high-density assays used in synapse-related neurobiological studies. (C) 2016 Elsevier Ltd. All rights reserved.
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Collections - College of Engineering > Department of Mechanical Engineering > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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