Sleep-promoting effects of a GABA/5-HTP mixture: Behavioral changes and neuromodulation in an invertebrate model
DC Field | Value | Language |
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dc.contributor.author | Hong, Ki-Bae | - |
dc.contributor.author | Park, Yooheon | - |
dc.contributor.author | Suh, Hyung Joo | - |
dc.date.accessioned | 2021-09-04T00:48:52Z | - |
dc.date.available | 2021-09-04T00:48:52Z | - |
dc.date.created | 2021-06-17 | - |
dc.date.issued | 2016-04-01 | - |
dc.identifier.issn | 0024-3205 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/88949 | - |
dc.description.abstract | Aims: This study was to investigate the sleep promoting effects of combined gamma-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP), by examining neuronal processes governing mRNA level alterations, as well as assessing neuromodulator concentrations, in a fruit fly model. Main methods: Behavioral assays were applied to investigate subjective nighttime activity, sleep episodes, and total duration of subjective nighttime sleep of two amino acids and GABA/5-HTP mixture with caffeine treated flies. Also, real-time PCR and HPLC analysis were applied to analyze the signaling pathway. Key findings: Subjective nighttime activity and sleep patterns of individual flies significantly decreased with 1% GABA treatment in conjunction with 0.1% 5-HTP treatment (p < 0.001). Furthermore, GABA/5-HTP mixture resulted in significant differences between groups related to sleep patterns (40%, p < 0.017) and significantly induced subjective nighttime sleep in the awake model (p < 0.003). These results related to transcript levels of the GABA(B) receptor (GABA(B)-R1) and serotonin receptor (5-HT1A), compared to the control group. In addition, GABA/5-HTP mixture significantly increased GABA levels 1 h and 12 h following treatment (2.1 fold and 1.2 fold higher than the control, respectively) and also increased 5-HTP levels (0 h: 1.01 mu g/protein, 12 h: 3.45 mu g/protein). Significance: In this regard, we successfully demonstrated that using a GABA/5-HTP mixture modulates subjective nighttime activity, sleep episodes, and total duration of subjective nighttime sleep to a greater extent than single administration of each amino acid, and that this modulation occurs via GABAergic and serotonergic signaling. (C) 2016 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.subject | INHIBITORY SYNAPTIC-TRANSMISSION | - |
dc.subject | GABA(B) RECEPTORS | - |
dc.subject | VIDEO-TRACKING | - |
dc.subject | DROSOPHILA | - |
dc.subject | SEROTONIN | - |
dc.subject | ACID | - |
dc.subject | 5-HYDROXYTRYPTOPHAN | - |
dc.subject | CAFFEINE | - |
dc.subject | BRAIN | - |
dc.subject | MODULATION | - |
dc.title | Sleep-promoting effects of a GABA/5-HTP mixture: Behavioral changes and neuromodulation in an invertebrate model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Suh, Hyung Joo | - |
dc.identifier.doi | 10.1016/j.lfs.2016.02.086 | - |
dc.identifier.scopusid | 2-s2.0-84961658151 | - |
dc.identifier.wosid | 000371790700007 | - |
dc.identifier.bibliographicCitation | LIFE SCIENCES, v.150, pp.42 - 49 | - |
dc.relation.isPartOf | LIFE SCIENCES | - |
dc.citation.title | LIFE SCIENCES | - |
dc.citation.volume | 150 | - |
dc.citation.startPage | 42 | - |
dc.citation.endPage | 49 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | INHIBITORY SYNAPTIC-TRANSMISSION | - |
dc.subject.keywordPlus | GABA(B) RECEPTORS | - |
dc.subject.keywordPlus | VIDEO-TRACKING | - |
dc.subject.keywordPlus | DROSOPHILA | - |
dc.subject.keywordPlus | SEROTONIN | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | 5-HYDROXYTRYPTOPHAN | - |
dc.subject.keywordPlus | CAFFEINE | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | MODULATION | - |
dc.subject.keywordAuthor | Insomnia | - |
dc.subject.keywordAuthor | Sleep | - |
dc.subject.keywordAuthor | gamma-Aminobutyric acid | - |
dc.subject.keywordAuthor | 5-Hydroxytryptophan | - |
dc.subject.keywordAuthor | Drosophila melanogaster | - |
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