Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Rhododenol and raspberry ketone impair the normal proliferation of melanocytes through reactive oxygen species-dependent activation of GADD45

Authors
Kim, MinjeongBaek, Heung SooLee, MiriPark, HyeonjiShin, Song SeokChoi, Dal WoongLim, Kyung-Min
Issue Date
4월-2016
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Keywords
Rhododenol; Raspberry ketone; B16F10 cell; Human melanocytes; GADD45
Citation
TOXICOLOGY IN VITRO, v.32, pp.339 - 346
Indexed
SCIE
SCOPUS
Journal Title
TOXICOLOGY IN VITRO
Volume
32
Start Page
339
End Page
346
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/89052
DOI
10.1016/j.tiv.2016.02.003
ISSN
0887-2333
Abstract
Rhododenol or rhododendrol (RD, 4-(4-hydroxyphenyl)-2-butanol) occurs naturally in many plants along with raspberry ketone (RK, 4-(4-hydroxyphenyl)-2-butanone), a ketone derivative, which include Nikko maple tree (Acer nikoense) and white birch (Betula platyphylla). De-pigmenting activity of RD was discovered and it was used as a brightening ingredient for the skin whitening cosmetics. Recently, cosmetics containing RD were withdrawn from the market because a number of consumers developed leukoderma, inflammation and erythema on their face, neck and hands. Here, we explored the mechanism underlying the toxicity of RD and RK against melanocytes using B16F10 murine melanoma cells and human primary epidermal melanocytes. Treatment with RD or RK resulted in the decreased cell viability in a dose-dependent manner which appeared from cell growth arrest Consistently, ROS generation was significantly increased by RD or RK as determined by DCF-enhanced fluorescence. An antioxidant enzyme, glutathione peroxidase was depleted as well. In line with ROS generation, oxidative damages and the arrest of normal cell proliferation, GADD genes (Growth Arrest and DNA Damage) that include GADD45 and GADD153, were significantly up-regulated. Prevention of ROS generation with an anti-oxidant, N-acetylcysteine (NAC) significantly rescued RD and RK-suppressed melanocyte proliferation. Consistently, up-regulation of GADD45 and GADD153 was significantly attenuated by NAC, suggesting that increased ROS and the resultant growth arrest of melanocytes may contribute to RD and RK-induced leukoderma. (C) 2016 Elsevier Ltd. All rights reserved.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Health Sciences > School of Health and Environmental Science > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE