Demineralized dentin matrix combined with recombinant human bone morphogenetic protein-2 in rabbit calvarial defects
- Authors
- Um, In-Woong; Hwang, Suk-Hyun; Kim, Young-Kyun; Kim, Moon-Young; Jun, Sang-Ho; Ryu, Jae-Jun; Jang, Hyon-Seok
- Issue Date
- 4월-2016
- Publisher
- KOREAN ACAD ORAL & MAXILLOFACIAL SURGERY
- Keywords
- Demineralized dentin matrix; Recombinant human bone morphogenetic protein-2; Microcomputed tomography; Histomorphometric analysis
- Citation
- JOURNAL OF THE KOREAN ASSOCIATION OF ORAL AND MAXILLOFACIAL SURGEONS, v.42, no.2, pp.90 - 98
- Indexed
- KCI
- Journal Title
- JOURNAL OF THE KOREAN ASSOCIATION OF ORAL AND MAXILLOFACIAL SURGEONS
- Volume
- 42
- Number
- 2
- Start Page
- 90
- End Page
- 98
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/89114
- DOI
- 10.5125/jkaoms.2016.42.2.90
- ISSN
- 2234-7550
- Abstract
- Objectives: The aim of this study was to compare the osteogenic effects of demineralized dentin matrix (DDM) combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) in rabbit calvarial defects with DDM and anorganic bovine bone (ABB) combined with rhBMP-2. Materials and Methods: Four round defects with 8-mm diameters were created in each rabbit calvaria. Each defect was treated with one of the following: 1) DDM, 2) ABB/rhBMP-2, or 3) DDM/rhBMP-2. The rhBMP-2 was combined with DDM and ABB according to a stepwise dry and dip lyophilizing protocol. Histological and microcomputed tomography (mu CT) analyses were performed to measure the amount of bone formation and bone volume after 2- and 8-week healing intervals. Results: Upon histological observation at two weeks, the DDM and ABB/rhBMP-2 groups showed osteoconductive bone formation, while the DDM/rhBMP-2 group showed osteoconductive and osteoinductive bone formation. New bone formation was higher in DDM/rhBMP-2, DDM and ABB decreasing order. The amounts of bone formation were very similar at two weeks; however, at eight weeks, the DDM/rhBMP-2 group showed a twofold greater amount of bone formation compared to the DDM and ABB/rhBMP-2 groups. The mu CT analysis showed markedly increased bone volume in the DDM/rhBMP-2 group at eight weeks compared with that of the DDM group. Notably, there was a slight decrease in bone volume in the ABB/rhBMP-2 group at eight weeks. There were no significant differences among the DDM, ABB/rhBMP-2, and DDM/rhBMP-2 groups at two or eight weeks. Conclusion: Within the limitations of this study, DDM appears to be a suitable carrier for rhBMP-2 in orthotopic sites.
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