Preparation of high purity docosahexaenoic acid from microalgae oil in a packed bed reactor via two-step lipase-catalysed esterification
DC Field | Value | Language |
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dc.contributor.author | Lee, Eun-Ju | - |
dc.contributor.author | Lee, Moon Won | - |
dc.contributor.author | No, Da Som | - |
dc.contributor.author | Kim, Hee-Jin | - |
dc.contributor.author | Oh, Se-Wook | - |
dc.contributor.author | Kim, Yangha | - |
dc.contributor.author | Kim, In-Hwan | - |
dc.date.accessioned | 2021-09-04T02:13:59Z | - |
dc.date.available | 2021-09-04T02:13:59Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2016-03 | - |
dc.identifier.issn | 1756-4646 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/89388 | - |
dc.description.abstract | Highly pure docosahexaenoic acid (DHA) was produced successfully in a packed bed reactor via the two-step lipase-catalysed esterification using the fatty acid from microalgae (from Crypthecodinium cohnii) and ethanol as substrates. Lipozyme RM IM from Rhizomucor miehei was employed as a biocatalyst. In the first step, a temperature of 40 degrees C, a molar ratio of 1:4 (fatty acid to ethanol), and a water content of 0.6 wt% (based on the total substrate weight) were selected as optimum conditions. A maximum DHA concentration of ca. 90 wt% was achieved in the fatty acid fraction with an 89 wt% yield after 15 min. In the second step, the fatty acids from the first step were used as the substrate and a recursive reaction was conducted using identical optimized conditions. Following this second step, a DHA concentration of 100 wt% was achieved in conjunction with an 83.2 wt% yield after 25 min. (C) 2015 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | POLYUNSATURATED FATTY-ACIDS | - |
dc.subject | IMMOBILIZED LIPASE | - |
dc.subject | WATER ACTIVITY | - |
dc.subject | TUNA OIL | - |
dc.subject | FISH-OIL | - |
dc.subject | MARINE | - |
dc.subject | PURIFICATION | - |
dc.subject | DHA | - |
dc.subject | TEMPERATURE | - |
dc.subject | ENRICHMENT | - |
dc.title | Preparation of high purity docosahexaenoic acid from microalgae oil in a packed bed reactor via two-step lipase-catalysed esterification | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, In-Hwan | - |
dc.identifier.doi | 10.1016/j.jff.2015.12.020 | - |
dc.identifier.scopusid | 2-s2.0-84957874391 | - |
dc.identifier.wosid | 000371281900033 | - |
dc.identifier.bibliographicCitation | JOURNAL OF FUNCTIONAL FOODS, v.21, pp.330 - 337 | - |
dc.relation.isPartOf | JOURNAL OF FUNCTIONAL FOODS | - |
dc.citation.title | JOURNAL OF FUNCTIONAL FOODS | - |
dc.citation.volume | 21 | - |
dc.citation.startPage | 330 | - |
dc.citation.endPage | 337 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalResearchArea | Nutrition & Dietetics | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Nutrition & Dietetics | - |
dc.subject.keywordPlus | POLYUNSATURATED FATTY-ACIDS | - |
dc.subject.keywordPlus | IMMOBILIZED LIPASE | - |
dc.subject.keywordPlus | WATER ACTIVITY | - |
dc.subject.keywordPlus | TUNA OIL | - |
dc.subject.keywordPlus | FISH-OIL | - |
dc.subject.keywordPlus | MARINE | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | DHA | - |
dc.subject.keywordPlus | TEMPERATURE | - |
dc.subject.keywordPlus | ENRICHMENT | - |
dc.subject.keywordAuthor | Crypthecodinium cohnii | - |
dc.subject.keywordAuthor | Docosahexaenoic acid (DHA) | - |
dc.subject.keywordAuthor | Esterification | - |
dc.subject.keywordAuthor | Lipase | - |
dc.subject.keywordAuthor | Microalgae | - |
dc.subject.keywordAuthor | Rhizomucor miehei | - |
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