Characterization and preparation of bio-tubular scaffolds for fabricating artificial vascular grafts by combining electrospinning and a co-culture system
- Authors
- Lee, Boram; Shafiq, Muhammad; Jung, Youngmee; Park, Jong-Chul; Kim, Soo Hyun
- Issue Date
- 2월-2016
- Publisher
- POLYMER SOC KOREA
- Keywords
- angiogenesis; endothelial cells; electrospinning; vascular scaffolds; PLCL
- Citation
- MACROMOLECULAR RESEARCH, v.24, no.2, pp.131 - 142
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- MACROMOLECULAR RESEARCH
- Volume
- 24
- Number
- 2
- Start Page
- 131
- End Page
- 142
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/89616
- DOI
- 10.1007/s13233-016-4017-5
- ISSN
- 1598-5032
- Abstract
- Tissue-engineered vascular scaffolds provide a promising solution for the replacement of diseased vascular structures. However, a major challenge lies in enhancing endothelialization, host cell ingrowth, and angiogenesis. In this study, we investigated the feasibility of developing a bio-tubular scaffold from human dermal fibroblasts (HDFs) and human umbilical vein endothelial cells (HUVEC) co-cultured on electrospun poly(L-lactide-co-epsilon-caprolactone) membranes to address these issues. Confluent layers of HDFs stimulated the organization of HUVECs into capillary-like networks in an indirect contact (two-dimensional) co-culture on membranes. Bio-tubular scaffolds fabricated from co-cultured membranes were either grown statically in vitro or implanted subcutaneously in severe combined immunodeficient mice for up to 4 weeks for biocompatibility evaluation and functional performance. In vitro examination of co-cultures on scaffolds showed collagen remodeling and an improvement in biomechanical properties up to day 14. Morphological analysis of in vitro grown bio-tubular scaffolds revealed good attachment and growth of both cell types. After one month, co-cultured scaffolds in vivo showed higher infiltration of host cells and collagen remodeling as compared to the HDF-seeded grafts. After 4 weeks, thin continuous layers of endothelial cells and smooth muscle cells were formed as shown by staining with an antibody specific for CD31and alpha-actin (alpha-SMA). On the contrary, HDF-seeded scaffolds remained free of alpha-SMA-positive cells at all time points, whereas few CD31(+) cells appeared after 4 weeks. Thus, co-cultured membranes provide a solution for enhancing endothelialization, tissue regeneration, and growth in bio-tubular scaffolds and may have broader applications in regenerative medicine.
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Collections - Graduate School > KU-KIST Graduate School of Converging Science and Technology > 1. Journal Articles
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