Effect of sustained release of rhBMP-2 from dried and wet hyaluronic acid hydrogel carriers compared with direct dip coating of rhBMP-2 on peri-implant osteogenesis of dental implants in canine mandibles
- Authors
- Pan, Hui; Han, Jeong Joon; Park, Yong-Doo; Cho, Tae Hyung; Hwang, Soon Jung
- Issue Date
- 2월-2016
- Publisher
- CHURCHILL LIVINGSTONE
- Keywords
- Peri-implant osteogenesis; Hyaluronic acid hydrogel; Dried coating; Dip coating; rhBMP-2
- Citation
- JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY, v.44, no.2, pp.116 - 125
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY
- Volume
- 44
- Number
- 2
- Start Page
- 116
- End Page
- 125
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/89644
- DOI
- 10.1016/j.jcms.2015.11.018
- ISSN
- 1010-5182
- Abstract
- Hyaluronic acid (HA) hydrogel has been used as a carrier of recombinant human bone morphogenetic protein (rhBMP)-2 for sustained delivery. To enhance peri-implant osteogenesis, a dried coating of rhBMP-2 HA hydrogel (BMP-HAH) on dental implants was designed; this approach provides the advantage of omitting in situ preparation of wet HA hydrogel. Sustained release of rhBMP-2 was more efficient for dried hydrogel over wet hydrogel. For both types, the released rhBMP-2 consistently led to enhanced alkaline phosphatase activity and osterix expression in human mesenchymal stromal cells. Histomorphometric analysis 4 weeks after placement of a dental implant in canine mandibles showed that the dried coating of BMP-HAH (10 mu g/ml, n = 6) resulted in a significantly greater bone area (BA) than the wet BMP-HAH (10 mu g/ml, n = 6) (p = 0.006) and implants without any coating (n = 6) (p = 0.022), while simple dip coating with rhBMP-2 (10 mu g/ml, n = 6) resulted in significantly greater BA than the other three groups (p < 0.0005). Bone-to-implant contact (BIC) was significantly different only between the dried and wet coating of BMP-HAH (p = 0.014). Our results suggest that a simple dip coating of rhBMP-2 is more effective for increased ped-implant osteogenesis compared to a coating of BMP-HAH with sustained release. (C) 2015 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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