Mitochondrial oxidative phosphorylation complexes exist in the sarcolemma of skeletal muscle
- Authors
- Lee, Hyun; Kim, Seung-Hyeob; Lee, Jae-Seon; Yang, Yun-Hee; Nam, Jwa-Min; Kim, Bong-Woo; Ko, Young-Gyu
- Issue Date
- 2월-2016
- Publisher
- KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
- Keywords
- Cytochrome c; Extracellular NADH-dependent respiration; Oxidative phosphorylation complexes; Sarcolemma; Skeletal muscle
- Citation
- BMB REPORTS, v.49, no.2, pp.116 - 121
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BMB REPORTS
- Volume
- 49
- Number
- 2
- Start Page
- 116
- End Page
- 121
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/89723
- DOI
- 10.5483/BMBRep.2016.49.2.232
- ISSN
- 1976-6696
- Abstract
- Although proteomic analyses have revealed the presence of mitochondrial oxidative phosphorylation (OXPHOS) proteins in the plasma membrane, there have been no in-depth evaluations of the presence or function of OXPHOS I-V in the plasma membrane. Here, we demonstrate the in situ localization of OXPHOS I-V complexes to the sarcolemma of skeletal muscle by immunofluorescence and immunohistochemistry. A portion of the OXPHOS I-V complex proteins was not co-stained with MitoTracker but co-localized with caveolin-3 in the sarcolemma of mouse gastrocnemius. Mitochondrial matrix-facing OXPHOS complex subunits were ectopically expressed in the sarcolemma of the non-permeabilized muscle fibers and C2C12 myotubes. The sarcolemmal localization of cytochrome c was also observed from mouse gastrocnemius muscles and C2C12 myotubes, as determined by confocal and total internal resonance fluorescence (TIRF) microscopy. Based on these data, we conclude that a portion of OXPHOS complexes is localized in the sarcolemma of skeletal muscle and may have non-canonical functions.
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