Chemopreventive Properties of Genipin on AGS Cell Line via Induction of JNK/Nrf2/ARE Signaling Pathway
- Authors
- Kim, Jee Min; Ko, Hyeonseok; Kim, Sun-Joong; Shim, So Hee; Ha, Chang Hoon; Chang, Hyo Ihl
- Issue Date
- Jan-2016
- Publisher
- WILEY
- Keywords
- Genipin; Nrf2; JNK; Reactive Oxygen Species; AGS Cells
- Citation
- JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, v.30, no.1, pp.45 - 54
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
- Volume
- 30
- Number
- 1
- Start Page
- 45
- End Page
- 54
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/89883
- DOI
- 10.1002/jbt.21741
- ISSN
- 1095-6670
- Abstract
- Roles of dietary phytochemicals in cancer chemoprevention via induction of nuclear factor-erythroid-2-related factor 2 (Nrf2)-mediated antioxidant enzymes have been well established in a number of studies. In this study, FACS analysis was used to reveal that the intracellular reactive oxygen species level decreased at 0-25 M of genipin treatment. Furthermore, immunofluorescence analysis and Western blotting were used to demonstrate that genipin treatment resulted in the upregulation and nuclear translocation of Nrf2, as well as upregulation of gastrointestinal glutathione peroxidase. Finally, we found that C-Jun-NH2-kinase (JNK) was also dose-dependently activated, where depleting JNK by using a biochemical inhibitor indicated that JNK was upstream of Nrf2. Interestingly, the antioxidant effects were limited to the treatment in the lower dosage of genipin, where higher dosage of genipin treatment resulted in the increased reactive oxygen species level and cytotoxicity. Thus, this study demonstrates for the first time that lower dosage of genipin results in the induction of JNK/Nrf2/ARE signaling pathway and protection from cell death.
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Collections - College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
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