Characterization of shear stress preventing red blood cells aggregation at the individual cell level: The temperature dependence
- Authors
- Lee, K.; Priezzhev, A.; Shin, S.; Yaya, F.; Meglinski, I.
- Issue Date
- 2016
- Publisher
- IOS PRESS
- Keywords
- single-cell level measurements; temperature; aggregation; critical shear stress; microfluidic flow; optical tweezers; Red blood cell; shearing-geometry
- Citation
- CLINICAL HEMORHEOLOGY AND MICROCIRCULATION, v.64, no.4, pp.853 - 857
- Indexed
- SCIE
SCOPUS
- Journal Title
- CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
- Volume
- 64
- Number
- 4
- Start Page
- 853
- End Page
- 857
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/90231
- DOI
- 10.3233/CH-168020
- ISSN
- 1386-0291
- Abstract
- BACKGROUND: The novel measure of the red blood cells (RBC) aggregation (RBC-A) -the critical (minimum) shear stress (CSS) to prevent the cells from aggregation was found to be a promising clinically significant parameter. However, the absolute values of this parameter were found to change significantly depending on the shearing geometry (cup-and-bob, cone-plate or microchannel-flow) and have different temperature dependences along with it. The direct confirmation of these dependences aimed to find out the correct values is still pending. OBJECTIVE: In this work, we aim to assess the absolute values of CSS at different temperatures. METHODS: The single cell level measurements of CSS were performed using optical tweezers. The measurements were carried out in heavily diluted suspensions of RBCs in plasma. RESULTS: The temperature dependent changes in CSS were measured at the points (22 and 38 degrees C), in which the cup-and-bob and cone-plate systems yielded about 1.5-fold different values, while the microchannel-flow system yielded a constant value. The single cell CSS were found to be 362 +/- 157mPa (22 degrees C) and 312 +/- 57mPa (38 degrees C). CONCLUSIONS: Our results prove that the microfluidic-flow approach is reflecting the RBC-A correctly. While the CSS values measured with other systems show the temperature dependent effect of the shearing geometry.
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Collections - College of Engineering > Department of Mechanical Engineering > 1. Journal Articles
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