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Use of phytic acid and hyper-salting to eliminate Escherichia coli O157:H7 from napa cabbage for kimchi production in a commercial plant

Authors
Kim, Nam HeeJang, Seong HoKim, Soon HanLee, Hee JungKim, YounghoonRyu, Jee HoonRhee, Min Suk
Issue Date
2-12월-2015
Publisher
ELSEVIER SCIENCE BV
Keywords
Salted napa cabbage; Escherichia coli O157:H7; Disinfection; Phytic acid; Sodium chloride
Citation
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, v.214, pp.24 - 30
Indexed
SCIE
SCOPUS
Journal Title
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volume
214
Start Page
24
End Page
30
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/91616
DOI
10.1016/j.ijfoodmicro.2015.07.024
ISSN
0168-1605
Abstract
The aim of this study was to develop a new salting method using natural phytic acid (PA) to ensure the microbiological safety and quality of salted napa cabbage used for kimchi production. The production of salted napa cabbage involves several stages: trimming, hyper-salting (20% NaCl) for up to 1 h, salting (10% NaCl for 10-18 h), three sequential washes in water (30 s for each), and draining (2 h). Two separate experiments were performed: one to determine the appropriate treatment conditions and a second to validate applicability under commercial conditions. In Experiment I, the effects of hyper-salting with PA on Escherichia coli O157:H7 numbers were tested in the laboratory. The following variables were monitored: 1) PA concentration (1, 2, 3%, w/w); 2) the ratio of the sample weight to the total volume of the solution (1:1.5,1:3, or 1:6); 3) the hyper-salting time (30 or 60 min); and 4) the salting time (2, 5, or 8 h). A procedure that achieved a >5-log reduction in the E. coli O157:H7 population was then tested in an actual kimchi processing plant (Experiment II). The results from Experiment I showed that bactericidal efficacy increased as all the measured variables increased (p < 0.05). Hyper-salting with 2% PA at a sample-to-water ratio (w/v) of 1:3 and 1:6 for 60 min resulted in a >5-log CFU/g reduction in the E. coli O157:H7 population. Further salting for 5 h completely eliminated (<1-log CFU/g) all bacteria. Thus, hyper-salting with PA 2% at a sample-to-water ratio of 1:3 for 60 min, followed by salting for 5 h, was tested under large-scale production conditions. The results revealed that the initial aerobic plate counts (APC), total coliform counts (TC), and fecal coliform counts (FC) were 6.6, 3.4, and 2.8-log CFU/g, respectively. The selected protocol reduced these values by 3.7-, >2.4-, and >1.8-log CFU/g, respectively. The 5 h salting step maintained the TC and FC at <1-log CFU/g; however, the APC recovered somewhat. The pH and salinity of the treated salted napa cabbages were within the ranges required for kimchi production (pH 5.1-5.3 and 1.5-2.0%, respectively). These results suggest that this novel method of salting food ensures microbiological safety and reduces the production time. (C) 2015 Elsevier B.V. All rights reserved.
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