Protective role of quercetin against cisplatin-induced hair cell damage in zebrafish embryos
- Authors
- Lee, S. K.; Oh, K. H.; Chung, A. Y.; Park, H. C.; Lee, S. H.; Kwon, S. Y.; Choi, J.
- Issue Date
- 11월-2015
- Publisher
- SAGE PUBLICATIONS LTD
- Keywords
- Quercetin; cisplatin; ototoxicity; zebrafish; hair cell
- Citation
- HUMAN & EXPERIMENTAL TOXICOLOGY, v.34, no.11, pp.1043 - 1052
- Indexed
- SCIE
SCOPUS
- Journal Title
- HUMAN & EXPERIMENTAL TOXICOLOGY
- Volume
- 34
- Number
- 11
- Start Page
- 1043
- End Page
- 1052
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/91965
- DOI
- 10.1177/0960327114567766
- ISSN
- 0960-3271
- Abstract
- Background and objectives: The aim of this study was to evaluate the protective effects of quercetin on cisplatin-induced hair cell damage in transgenic zebrafish embryos. Materials and methods: Five days postfertilization zebrafish embryos were exposed to 1 mM cisplatin and quercetin at 10, 50, 100, or 200 M for 4 h. Hair cells within neuromasts of the supraorbital, otic, and occipital lateral lines were analyzed by fluorescent microscopy (n = 10). Survival of hair cells was calculated as the average number of hair cells in the control group that were not exposed to cisplatin. Ultrastructural changes were evaluated using scanning electron microscopy. Results: Hair cell damage in neuromasts was decreased by co-treatment of quercetin and cisplatin (quercetin 100 M: 8.6 1.1 cells; 1 mM cisplatin only: 5.0 +/- 0.5 cells; n = 10, p < 0.05); apoptosis of hair cells examined by special stain was also decreased by quercetin. The ultrastructure of hair cells within neuromasts was preserved in zebrafish by the combination of quercetin (100 M) and cisplatin (1 mM). Conclusion: In conclusion, quercetin showed protective effects against cisplatin-induced toxicity in a zebrafish model. The results of this study suggest the possibility of a protective role of quercetin against cisplatin-induced apoptotic cell death in zebrafish.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
- College of Medicine > Department of Medical Science > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.