Chemiluminometric Immunosensor for High-Sensitivity Cardiac Troponin I Employing a Polymerized Enzyme Conjugate as a Tracer
- Authors
- Lim, Guei-Sam; Seo, Sung-Min; Paek, Sung-Ho; Kim, Seung-Wan; Jeon, Jin-Woo; Kim, Dong-Hyung; Cho, Il-Hoon; Paek, Se-Hwan
- Issue Date
- 7-10월-2015
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- SCIENTIFIC REPORTS, v.5
- Indexed
- SCIE
SCOPUS
- Journal Title
- SCIENTIFIC REPORTS
- Volume
- 5
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/92208
- DOI
- 10.1038/srep14848
- ISSN
- 2045-2322
- Abstract
- To detect high-sensitivity cardiac troponin I (hs-cTnI; <0.01 ng/mL) at points of care, we developed a rapid immunosensor by using horseradish peroxidase polymerized in 20 molecules on average (Poly-HRP) as a tracer conjugated with streptavidin (SA-Poly-HRP). As shown in the conventional system, enhanced sensitivity could be achieved by using a sequential binding scheme for the complex formation to contain the huge molecular tracer. We used a 2-dimensional chromatographic technology to carry out the sequential bindings in cross-flow directions. After the complex formation of antigen-antibody with analyte in a vertical direction, SA-Poly-HRP was horizontally supplied across the membrane strip for additional binding via a biotin-SA linkage. The HRP substrate was subsequently supplied along the same direction to produce a chemiluminometric signal, which was measured by a cooled charge-coupled device. Hs-cTnI analysis was completed in this format within 25 min, and the results showed a high correlation with those of the CentaurXP (R) reference system (R-2 > 0.99). The detection limit of the rapid immunosensor was 0.003 +/- 0.001 ng/mL cTnI, corresponding to a 10-fold improvement compared to results using the plain enzyme tracer. This demonstrated the measurement of hs-cTnI in a much more cost-effective manner compared to the automated versions currently available.
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Collections - College of Science and Technology > Department of Biotechnology and Bioinformatics > 1. Journal Articles
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