Comparison of the Diversity of Basidiomycetes from Dead Wood of the Manchurian fir (Abies holophylla) as Evaluated by Fruiting Body Collection, Mycelial Isolation, and 454 Sequencing
- Authors
- Jang, Yeongseon; Jang, Seokyoon; Min, Mihee; Hong, Joo-Hyun; Lee, Hanbyul; Lee, Hwanhwi; Lim, Young Woon; Kim, Jae-Jin
- Issue Date
- 10월-2015
- Publisher
- SPRINGER
- Keywords
- Dead wood; Mycoparasitic fungi; Mycorrhizal fungi; Wood-decay fungi
- Citation
- MICROBIAL ECOLOGY, v.70, no.3, pp.634 - 645
- Indexed
- SCIE
SCOPUS
- Journal Title
- MICROBIAL ECOLOGY
- Volume
- 70
- Number
- 3
- Start Page
- 634
- End Page
- 645
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/92287
- DOI
- 10.1007/s00248-015-0616-5
- ISSN
- 0095-3628
- Abstract
- In this study, three different methods (fruiting body collection, mycelial isolation, and 454 sequencing) were implemented to determine the diversity of wood-inhabiting basidiomycetes from dead Manchurian fir (Abies holophylla). The three methods recovered similar species richness (26 species from fruiting bodies, 32 species from mycelia, and 32 species from 454 sequencing), but Fisher's alpha, Shannon-Wiener, Simpson's diversity indices of fungal communities indicated fruiting body collection and mycelial isolation displayed higher diversity compared with 454 sequencing. In total, 75 wood-inhabiting basidiomycetes were detected. The most frequently observed species were Heterobasidion orientale (fruiting body collection), Bjerkandera adusta (mycelial isolation), and Trichaptum fusco-violaceum (454 sequencing). Only two species, Hymenochaete yasudae and Hypochnicium karstenii, were detected by all three methods. This result indicated that Manchurian fir harbors a diverse basidiomycetous fungal community and for complete estimation of fungal diversity, multiple methods should be used. Further studies are required to understand their ecology in the context of forest ecosystems.
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Collections - College of Life Sciences and Biotechnology > Division of Environmental Science and Ecological Engineering > 1. Journal Articles
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