Evolutionary engineering of Saccharomyces cerevisiae for efficient conversion of red algal biosugars to bioethanol
- Authors
- Lee, Hye-Jin; Kim, Soo-Jung; Yoon, Jeong-Jun; Kim, Kyoung Heon; Seo, Jin-Ho; Park, Yong-Cheol
- Issue Date
- Sep-2015
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Red algae; Galactose; Bioethanol; Saccharomyces cerevisiae; Evolutionary engineering
- Citation
- BIORESOURCE TECHNOLOGY, v.191, pp.445 - 451
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 191
- Start Page
- 445
- End Page
- 451
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/92630
- DOI
- 10.1016/j.biortech.2015.03.057
- ISSN
- 0960-8524
- Abstract
- The aim of this work was to apply the evolutionary engineering to construct a mutant Saccharomyces cerevisiae HJ7-14 resistant on 2-deoxy-D-glucose and with an enhanced ability of bioethanol production from galactose, a mono-sugar in red algae. In batch and repeated-batch fermentations, HJ7-14 metabolized 5-fold more galactose and produced ethanol 2.1-fold faster than the parental D452-2 strain. Transcriptional analysis of genes involved in the galactose metabolism revealed that moderate relief from the glucose-mediated repression of the transcription of the GAL genes might enable HJ7-14 to metabolize galactose rapidly. HJ7-14 produced 7.4 g/L ethanol from hydrolysates of the red alga Gelidium amansii within 12 h, which was 1.5-times faster than that observed with D452-2. We demonstrate conclusively that evolutionary engineering is a promising tool to manipulate the complex galactose metabolism in S. cerevisiae to produce bioethanol from red alga. (C) 2015 Elsevier Ltd. All rights reserved.
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