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Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase

Authors
Baik, Joo HyunShin, Kwang-SoonPark, YooheonYu, Kwang-WonSuh, Hyung JooChoi, Hyeon-Son
Issue Date
30-8월-2015
Publisher
WILEY
Keywords
simultaneous enzymatic process; biotransformation; tea polysaccharide; radical scavenging activity; IL-6 production
Citation
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, v.95, no.11, pp.2337 - 2344
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
Volume
95
Number
11
Start Page
2337
End Page
2344
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/92727
DOI
10.1002/jsfa.6955
ISSN
0022-5142
Abstract
BACKGROUNDGreen tea is a dietary source of bioactive compounds for human health. Enzymatic treatments induce the bioconversion of bioactive components, which can improve biological activities. In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE). RESULTSTannase and pectinase treatments induced the biotransformation of catechins and altered tea polysaccharide () content. The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 mu g mL(-1)) and a reduction in epigallocatechin gallate (EGCG). Biotransformation of catechins improved the radical scavenging activity of GTE. Pectinase treatment led to change of TPS composition in GTE by hydrolyzing polysaccharides. In addition, pectinase-driven hydrolysis in polysaccharides significantly increased TPS-induced Interleukin 6 (IL-6) production in macrophages. In particular, treatment of Rapidase (TPS-Ra) led to the highest IL-6 production among TPS samples, similar to treatment of highly purified pectinase (TPS-GTE), a positive control. CONCLUSIONSimultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea. (c) 2014 Society of Chemical Industry
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보건과학대학 (바이오시스템의과학부)
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