Mitogen activated protein kinase pathway-dependent effects of platelet-derived growth factor on migration of trophectoderm cells
- Authors
- Jeong, Wooyoung; Song, Gwonhwa; Kim, Jinyoung
- Issue Date
- 7-Aug-2015
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- PDGF; Trophectoderm; Migration; Peri-implantation
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.463, no.4, pp.575 - 581
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 463
- Number
- 4
- Start Page
- 575
- End Page
- 581
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/92772
- DOI
- 10.1016/j.bbrc.2015.05.098
- ISSN
- 0006-291X
- Abstract
- Successful development of the conceptus and implantation requires an intimate trophic connection between maternal uterus and conceptus mediated by local regulators including growth factors. Platelet-derived growth factor (PDGF) acts as a chemotactic factor for a variety of cell types. Current studies have determined that PDGF participates in rapid growth and development of cleavage stage embryos, but PDGF-induced effects on the growth and development of pen-implantation conceptus remains unknown. In the present study, PDGF induced phosphorylation of ERK1/2, ART and RPS6 proteins in porcine trophectoderm (pTr) cells in a dose- and time-dependent manner. Addition of U0126 (an inhibitor of ERK1/2) or LY294002 (a PI3K inhibitor) blocked PDGF-induced effects on phosphorylation of signaling proteins. Combinations of PDGF and U0126 decreased PDGF-induced p-ERK1/2 and p-AKT1, but combinations of PDGF and LY294002 blocked only PDGF-induced ART phosphorylation. Furthermore, PDGF significantly induced pTr cell migration and these stimulatory effects were blocked by U0126 and LY294002. Immunoreactive p-ERK1/2 and p-RPS6 proteins were abundant in pTr cells treated with PDGF, but U0126 reduced PDGF-induced p-ERK1/2 and p-RPS6 levels to basal amounts. Present study suggests that PDGF secreted into the maternal-conceptus microenvironment stimulates pTr cell migration through signal transduction cascades mediated by the ERK1/2 MAPK and AKT1 pathways. (C) 2015 Elsevier Inc. All rights reserved.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
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