Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Functional characterization and reconstitution of ABA signaling components using transient gene expression in rice protoplasts

Authors
Kim, NamhyoMoon, Seok-JunMin, Myung K.Choi, Eun-HyeKim, Jin-AeKoh, Eun Y.Yoon, InsunByun, Myung-OkYoo, Sang-DongKim, Beom-Gi
Issue Date
5-8월-2015
Publisher
FRONTIERS MEDIA SA
Keywords
ABA; rice protoplast; reconstitution of signaling; transient expression; dual luciferase assay
Citation
FRONTIERS IN PLANT SCIENCE, v.6
Indexed
SCIE
SCOPUS
Journal Title
FRONTIERS IN PLANT SCIENCE
Volume
6
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/92777
DOI
10.3389/fpls.2015.00614
ISSN
1664-462X
Abstract
The core components of ABA-dependent gene expression signaling have been identified in Arabidopsis and rice. This signaling pathway consists of four major components; group A OsbZIPs, SAPKs, subclass A OsPP2Cs and OsPYL/RCARs in rice. These might be able to make thousands of combinations through interaction networks resulting in diverse signaling responses. We tried to characterize those gene functions using transient gene expression for rice protoplasts (TGERP) because it is instantaneous and convenient system. Firstly, in order to monitor the ABA signaling output, we developed reporter system named pRabl6A-fLUC which consists of Rab16A promoter of rice and luciferase gene. It responses more rapidly and sensitively to ABA than pABRC3-fLUC that consists of ABRC3 of HVA1 promoter in TGERP. We screened the reporter responses for over-expression of each signaling components from group A OsbZIPs to OsPYL/RCARs with or without ABA in TGERP. OsbZIP46 induced reporter most strongly among OsbZIPs tested in the presence of ABA. SAPKs could activate the OsbZIP46 even in the ABA independence. Subclass A OsPP2C6 and -8 almost completely inhibited the OsbZIP46 activity in the different degree through the SAPK9. Lastly, OsPYL/RCAR2 and -5 rescued the OsbZIP46 activity in the presence of SAPK9 and OsPP2C6 dependent on ABA concentration and expression level. By using TGERP, we could characterize successfully the effects of ABA dependent gene expression signaling components in rice. In conclusion, TGERP represents very useful technology to study systemic functional genomics in rice or other monocots.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Life Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE