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Three novel clade B serine protease inhibitors from disk abalone, Haliotis discus discus: Molecular perspectives and responses to immune challenges and tissue injury

Authors
Bathige, S. D. N. K.Umasuthan, NavaneethaiyerGodahewa, G. I.Whang, IlsonKim, ChulPark, Hae-ChulLee, Jehee
Issue Date
8월-2015
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Keywords
Serine protease inhibitor (SERPIN); Haliotis discus discus; Immune response; Tissue injury
Citation
FISH & SHELLFISH IMMUNOLOGY, v.45, no.2, pp.334 - 341
Indexed
SCIE
SCOPUS
Journal Title
FISH & SHELLFISH IMMUNOLOGY
Volume
45
Number
2
Start Page
334
End Page
341
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/92805
DOI
10.1016/j.fsi.2015.04.020
ISSN
1050-4648
Abstract
Serine protease inhibitors (SERPINs) control cellular protease activity in order to maintain cellular homeostasis. The immune and inflammatory responses of invertebrate clade B SERPINs have not been widely reported. In the present study, three proteins with high similarity to clade B SERPINs, referred to as AbSERPIN-1, AbSERPIN-2 and AbSERPIN-3, were identified from disk abalone (Haliotis discus discus). While AbSERPIN-1 (399 aa) was of a typical size for this protein class, AbSERPIN-2 (506 aa) and AbSERPIN-3 (532 aa) were relatively larger. Bioinformatic analysis revealed the characteristic SERPIN domain in each AbSERPIN. In addition, the N-terminal region of both AbSERPIN-2 and AbSERPIN-3 contained a predicted low complexity region (LCR) and a signal peptide, suggesting that these proteins are secretory proteins and are, thus, novel peptides. Tertiary structural models of the AbSERPINs highlighted their structural and functional conservation. Ubiquitous expression of AbSERPIN transcripts was evaluated by quantitative real time PCR (qPCR) analysis in seven tissue types. AbSERPIN-1, AbSERPIN-2, and AbSERPIN-3 transcript levels were highest in mantle, hemocytes, and muscles, respectively. Temporal expression analysis revealed that AbSERPINs were significantly (P < 0.05) elevated in hemocytes during the early/middle stages following the injection of a bacterial pathogen (Vibrio parahaemolyticus or Listeria monocytogenes) or an immuno-stimulant (lipopolysaccharide). Moreover, mantle tissue injury led to significant changes in the temporal expression of AbSERPIN mRNA. Specifically, transcription of AbSERPIN-1 and AbSERPIN-3 was considerably up-regulated, while expression of AbSERPIN-2 was suppressed. These results suggest a potential role of AbSERPINs in response to pathogen invasion and tissue injury in disk abalone. (C) 2015 Elsevier Ltd. All rights reserved.
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