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Anti-hyperglycemic activity of polyphenols isolated from barnyard millet (Echinochloa utilis L.) and their role inhibiting alpha-glucosidase

Authors
Seo, Kyung-HyeRa, Ji-EunLee, Sung-JoonLee, Ji HaeKim, Sun RimLee, Jin HwanSeo, Woo Duck
Issue Date
8월-2015
Publisher
KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY
Keywords
alpha-Glucosidase; Anti-hyperglycemic; Barnyard millet grains; Caco-2 cells; Serotonin
Citation
JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, v.58, no.4, pp.571 - 579
Indexed
SCIE
SCOPUS
KCI
Journal Title
JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY
Volume
58
Number
4
Start Page
571
End Page
579
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/92935
DOI
10.1007/s13765-015-0070-6
ISSN
1738-2203
Abstract
The extracts and chemical compounds isolated from barnyard millet (Echinochloa utilis) grains were investigated as part of a search for naturally derived anti-hyperglycemic medicinal plants. Among the five different solvent extracts, the ethyl acetate extract showed the lowest IC50 value against Saccharomyces cerevisiae alpha-glucosidase (70.2 mu g/mL). Chromatography yielded eight phenolic compounds that may have been responsible for this effect. Among them, N-p-coumaroyl serotonin (1, CS), feruloyl serotonin (2, FS), and luteolin (5) potently inhibited alpha-glucosidase with IC50 values of 1.3-17.8 mu M compared with those of deoxynojirimycin (DNJ, IC50 = 2.5 +/- 0.1 mu M) and acarbose (IC50 = 255.1 +/- 15.6 mu M). Additionally, for the first time, we found that CS and FS were significantly inhibited mammalian rat intestinal sucrase (IC50 of 3.0 and 8.2 mu M) and reduced glucose content (73 and 52 % at 0.1 mg/wells) in Caco-2 (human intestinal epithelial) cells. Furthermore, oral glucose tolerance test revealed improved glucose tolerance following treatment with the barnyard millet grains extract by retarding the postprandial rise in blood glucose in vivo. These results suggest that barnyard millet grain can be used as a natural functional medicine to prevent and alleviate type-2 diabetes.
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