Phase-Contrast Radiography Enables Detection of Early Changes in Articular Cartilage in a Mouse Model of Osteoarthritis
- Authors
- Yoon, Joon Shik; Won, Sun Jae; Kim, Sei Joo; Hong, Suk Joo; Kim, Gui Sang; Kim, Jae Min
- Issue Date
- 8월-2015
- Publisher
- LIPPINCOTT WILLIAMS & WILKINS
- Keywords
- Animal Model; Cartilage; Osteoarthritis; Phase-Contrast; Tomography
- Citation
- AMERICAN JOURNAL OF PHYSICAL MEDICINE & REHABILITATION, v.94, no.8, pp.644 - 648
- Indexed
- SCIE
SCOPUS
- Journal Title
- AMERICAN JOURNAL OF PHYSICAL MEDICINE & REHABILITATION
- Volume
- 94
- Number
- 8
- Start Page
- 644
- End Page
- 648
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/92941
- DOI
- 10.1097/PHM.0000000000000232
- ISSN
- 0894-9115
- Abstract
- Objective The aim of this study was to investigate the potential of using phase-contrast radiography to detect early changes in articular cartilage in a mouse model of osteoarthritis. Design An iodoacetate-induced mouse model of osteoarthritis was used. Mice with iodoacetate-induced osteoarthritis were euthanized at 1 and 2 wks after injection. Specimens were obtained by sectioning mouse tissue into 5-mm-thick slices. Sections were examined using synchrotron x-rays from the 7B2 beamline at the Pohang Light Source. Magnified microradiographic images, obtained by phase-contrast radiography, were used to identify changes in articular cartilage and the subchondral bone. Results Refraction-enhanced phase-contrast radiography enabled detailed microscopic and macroscopic observation of articular cartilage by a noninvasive technique. Detailed microscopic images revealed partially damaged articular cartilage, containing an irregular trilaminar zone, at 1 wk after injection. At 2 wks after injection, a necrotic cleft was clearly visible, along with collapse of the trilaminar zones. Shrunken chondrocytes and chondrocyte clusters of variable sizes were also observed. Conclusions Phase-contrast radiography is a feasible technique for the early detection of osteoarthritis and is capable of detecting microstructural changes in articular cartilage.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
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