Concave microwell array-mediated three-dimensional tumor model for screening anticancer drug-loaded nanoparticles
DC Field | Value | Language |
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dc.contributor.author | Kang, AhRan | - |
dc.contributor.author | Seo, Hye In | - |
dc.contributor.author | Chung, Bong Geun | - |
dc.contributor.author | Lee, Sang-Hoon | - |
dc.date.accessioned | 2021-09-04T14:30:57Z | - |
dc.date.available | 2021-09-04T14:30:57Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2015-07 | - |
dc.identifier.issn | 1549-9634 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/93107 | - |
dc.description.abstract | We investigated the effect of anticancer drug-loaded functional polymeric nanoparticles on drug resistance of three-dimensional (3D) breast tumor spheroids. 3D tumor models were built using concave microwells with different diameters (300-700 mu m) and nanoparticles were prepared using thermo-responsive poly(N-isopropylacrylamide) (PNIPAM)-co-acrylic acid (AA). Upon culturing with doxorubicin-loaded PNIPAM-co-AA nanoparticles for 96 hours, the smallest tumor spheroids were extensively disrupted, resulting in a reduction in spheroid diameter. In contrast, the sizes of the largest tumor spheroids were not changed. Scanning electron microscopy revealed that the circular shape of 3D spheroids treated with doxorubicin-loaded PNIPAM-co-AA nanoparticles had collapsed severely. Cell viability assays also demonstrated that the largest tumor spheroids cultured with doxorubicin-loaded PNIPAM-co-AA nanoparticles were highly resistant to the anticancer drug. We confirmed that tight cell-cell contacts within largest tumor spheroids significantly improved the anticancer drug resistance. Therefore, this uniform-sized 3D breast tumor model could be a potentially powerful tool for anticancer drug screening applications. From the Clinical Editor: The battle against cancer is a big challenge. With new anti-cancer drugs being developed under the nanotechnology platform, there is a need to have a consistent and reliable testing system that mimics the in-vivo tumor scenario. The authors successfully designed a 3D tumor model using concave microwells to produce different tumor diameters. This will be of value for future drug screening. (C) 2015 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | MULTICELLULAR SPHEROIDS | - |
dc.subject | CELL | - |
dc.subject | CULTURE | - |
dc.subject | 3D | - |
dc.subject | GENERATION | - |
dc.subject | 3-D | - |
dc.title | Concave microwell array-mediated three-dimensional tumor model for screening anticancer drug-loaded nanoparticles | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Sang-Hoon | - |
dc.identifier.doi | 10.1016/j.nano.2015.02.009 | - |
dc.identifier.scopusid | 2-s2.0-84931026792 | - |
dc.identifier.wosid | 000363967100012 | - |
dc.identifier.bibliographicCitation | NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, v.11, no.5, pp.1153 - 1161 | - |
dc.relation.isPartOf | NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | - |
dc.citation.title | NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | - |
dc.citation.volume | 11 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1153 | - |
dc.citation.endPage | 1161 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | MULTICELLULAR SPHEROIDS | - |
dc.subject.keywordPlus | CELL | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | 3D | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | 3-D | - |
dc.subject.keywordAuthor | Three-dimensional tumor model | - |
dc.subject.keywordAuthor | Anticancer drug screening | - |
dc.subject.keywordAuthor | Polymeric nanoparticle | - |
dc.subject.keywordAuthor | Concave microwell array | - |
dc.subject.keywordAuthor | Uniform-sized tumor | - |
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