Concave microwell array-mediated three-dimensional tumor model for screening anticancer drug-loaded nanoparticles
- Authors
- Kang, AhRan; Seo, Hye In; Chung, Bong Geun; Lee, Sang-Hoon
- Issue Date
- 7월-2015
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Three-dimensional tumor model; Anticancer drug screening; Polymeric nanoparticle; Concave microwell array; Uniform-sized tumor
- Citation
- NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, v.11, no.5, pp.1153 - 1161
- Indexed
- SCIE
SCOPUS
- Journal Title
- NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
- Volume
- 11
- Number
- 5
- Start Page
- 1153
- End Page
- 1161
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/93107
- DOI
- 10.1016/j.nano.2015.02.009
- ISSN
- 1549-9634
- Abstract
- We investigated the effect of anticancer drug-loaded functional polymeric nanoparticles on drug resistance of three-dimensional (3D) breast tumor spheroids. 3D tumor models were built using concave microwells with different diameters (300-700 mu m) and nanoparticles were prepared using thermo-responsive poly(N-isopropylacrylamide) (PNIPAM)-co-acrylic acid (AA). Upon culturing with doxorubicin-loaded PNIPAM-co-AA nanoparticles for 96 hours, the smallest tumor spheroids were extensively disrupted, resulting in a reduction in spheroid diameter. In contrast, the sizes of the largest tumor spheroids were not changed. Scanning electron microscopy revealed that the circular shape of 3D spheroids treated with doxorubicin-loaded PNIPAM-co-AA nanoparticles had collapsed severely. Cell viability assays also demonstrated that the largest tumor spheroids cultured with doxorubicin-loaded PNIPAM-co-AA nanoparticles were highly resistant to the anticancer drug. We confirmed that tight cell-cell contacts within largest tumor spheroids significantly improved the anticancer drug resistance. Therefore, this uniform-sized 3D breast tumor model could be a potentially powerful tool for anticancer drug screening applications. From the Clinical Editor: The battle against cancer is a big challenge. With new anti-cancer drugs being developed under the nanotechnology platform, there is a need to have a consistent and reliable testing system that mimics the in-vivo tumor scenario. The authors successfully designed a 3D tumor model using concave microwells to produce different tumor diameters. This will be of value for future drug screening. (C) 2015 Elsevier Inc. All rights reserved.
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Collections - College of Health Sciences > School of Biomedical Engineering > 1. Journal Articles
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