Synthesis of trans-10,cis-12 conjugated linoleic acid-enriched triacylglycerols via two-step lipase-catalyzed esterification
DC Field | Value | Language |
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dc.contributor.author | Kang, Ingu | - |
dc.contributor.author | Bang, Hyo-Jeong | - |
dc.contributor.author | Kim, In-Hwan | - |
dc.contributor.author | Choi, Hee-Don | - |
dc.contributor.author | Kim, Byung Hee | - |
dc.date.accessioned | 2021-09-04T15:26:07Z | - |
dc.date.available | 2021-09-04T15:26:07Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2015-06 | - |
dc.identifier.issn | 0023-6438 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/93357 | - |
dc.description.abstract | This study aimed to synthesize trans-10,cis-12 conjugated linoleic acid (t10,c12-CIA)-enriched tri-acylglycerols (TAGs) with potential anti-obesity effects via a two-step lipase-catalyzed reaction. Commercial CLA isomer mixtures, containing 33.3 g/100 g t10,c12-CLA, were esterified with dodecan-1-ol to selectively enrich the t10,c12-CLA in a free fatty acid (FFA) fraction. The reaction was performed in a recirculating packed bed reactor using Candida rugosa lipase (immobilized on Immobead 150) as the biocatalyst. An FFA fraction containing 54.7 g/100 g t10,c12-CLA was produced in a yield of 21.8 g/100 g initial t10,c12-CLA under the optimal conditions, i.e., temperature, 20 degrees C; CLA mixtures-to-dodecan-1-ol molar ratio, 1:1; water content, zero (no added water); reaction time, 36 h. A t10,c12-CLA-enriched FFA fraction was esterified with glycerol to prepare t10,c12-CLA-enriched TAGs. The reaction was performed in a stirred batch reactor using Candid antarctica lipase B (immobilized on macroporous acrylic resin) as the biocatalyst. The optimal combination of temperature, glycerol-to-FFA fraction molar ratio, enzyme loading, reaction time, and vacuum level was 60 degrees C, 1:3, 10 g/100 g (based on total substrates), 12 h, and 0.4 kPa, respectively. Under these conditions, the TAG content reached 93.7 g/100 g and t10,c12-CLA was evenly distributed throughout the glycerol backbone of the TAG. (C) 2015 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | CANDIDA-RUGOSA LIPASE | - |
dc.subject | SELECTIVE ESTERIFICATION | - |
dc.subject | BODY-WEIGHT | - |
dc.subject | CLA ISOMERS | - |
dc.subject | FRACTIONATION | - |
dc.subject | SOLVENT | - |
dc.subject | HUMANS | - |
dc.subject | LIPIDS | - |
dc.subject | TAG | - |
dc.title | Synthesis of trans-10,cis-12 conjugated linoleic acid-enriched triacylglycerols via two-step lipase-catalyzed esterification | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, In-Hwan | - |
dc.identifier.doi | 10.1016/j.lwt.2015.01.041 | - |
dc.identifier.scopusid | 2-s2.0-84923222082 | - |
dc.identifier.wosid | 000351645800035 | - |
dc.identifier.bibliographicCitation | LWT-FOOD SCIENCE AND TECHNOLOGY, v.62, no.1, pp.249 - 256 | - |
dc.relation.isPartOf | LWT-FOOD SCIENCE AND TECHNOLOGY | - |
dc.citation.title | LWT-FOOD SCIENCE AND TECHNOLOGY | - |
dc.citation.volume | 62 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 249 | - |
dc.citation.endPage | 256 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | CANDIDA-RUGOSA LIPASE | - |
dc.subject.keywordPlus | SELECTIVE ESTERIFICATION | - |
dc.subject.keywordPlus | BODY-WEIGHT | - |
dc.subject.keywordPlus | CLA ISOMERS | - |
dc.subject.keywordPlus | FRACTIONATION | - |
dc.subject.keywordPlus | SOLVENT | - |
dc.subject.keywordPlus | HUMANS | - |
dc.subject.keywordPlus | LIPIDS | - |
dc.subject.keywordPlus | TAG | - |
dc.subject.keywordAuthor | Esterification | - |
dc.subject.keywordAuthor | Lipase | - |
dc.subject.keywordAuthor | Recirculating packed bed reactor | - |
dc.subject.keywordAuthor | Triacylglycerol | - |
dc.subject.keywordAuthor | trans-10,cis-12 conjugated linoleic acid | - |
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