Low-cost telemedicine device performing cell and particle size measurement based on lens-free shadow imaging technology
DC Field | Value | Language |
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dc.contributor.author | Roy, Mohendra | - |
dc.contributor.author | Seo, Dongmin | - |
dc.contributor.author | Oh, Chang-Hyun | - |
dc.contributor.author | Nam, Myung-Hyun | - |
dc.contributor.author | Kim, Young Jun | - |
dc.contributor.author | Seo, Sungkyu | - |
dc.date.accessioned | 2021-09-04T16:05:43Z | - |
dc.date.available | 2021-09-04T16:05:43Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2015-05-15 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/93557 | - |
dc.description.abstract | Recent advances in lens-free shadow imaging technology have enabled a new class of cell imaging platform, which is a suitable candidate for point-of-care facilities. In this paper, we firstly demonstrate a compact and low-cost telemedicine device providing automated cell and particle size measurement based on lens-free shadow imaging technology. Using the generated shadow (or diffraction) patterns, the proposed approach can detect and measure the sizes of more than several hundreds of micro-objects simultaneously within a single digital image frame. In practical experiments, we defined four types of shadow parameters extracted from each micro-object shadow pattern, and found that a specific shadow parameter (peak-to-peak distance, PPD) demonstrated a linear relationship with the actual micro-object sizes. By using this information, a new algorithm suitable for operation on both a personal computer (PC) and a cell phone was also developed, providing automated size detection of poly-styrenemicro-beads and biological cells such as red blood cells, MCF-7, HepG2, and HeLa. Results from the proposed device were compared with those of a conventional optical microscope, demonstrating good agreement between two approaches. In contrast to other existing cell and particle size measurement approaches, such as Coulter counter, flow-cytometer, particle-size analyzer, and optical microscope, this device can provide accurate cell and particle size information with a 2 mu m maximum resolution, at almost no cost (less than 100 USD), within a compact instrumentation size (9.3 x 9.0 x 9.0 cm(3)), and in a rapid manner (within 1 min). The proposed lens-free automated particle and cell size measurement device, based on shadow imaging technology, can be utilized as a powerful tool for many cell and particle handling procedures, including environmental, pharmaceutical, biological, and clinical applications. (C) 2014 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Low-cost telemedicine device performing cell and particle size measurement based on lens-free shadow imaging technology | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Chang-Hyun | - |
dc.contributor.affiliatedAuthor | Nam, Myung-Hyun | - |
dc.contributor.affiliatedAuthor | Kim, Young Jun | - |
dc.contributor.affiliatedAuthor | Seo, Sungkyu | - |
dc.identifier.doi | 10.1016/j.bios.2014.10.040 | - |
dc.identifier.scopusid | 2-s2.0-84922330627 | - |
dc.identifier.wosid | 000350076900104 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.67, pp.715 - 723 | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 67 | - |
dc.citation.startPage | 715 | - |
dc.citation.endPage | 723 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordAuthor | Cell size measurement | - |
dc.subject.keywordAuthor | Lens-free imaging | - |
dc.subject.keywordAuthor | Shadow | - |
dc.subject.keywordAuthor | Diffraction | - |
dc.subject.keywordAuthor | CMOS image sensor | - |
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