CXCR2 and Its Related Ligands Play a Novel Role in Supporting the Pluripotency and Proliferation of Human Pluripotent Stem Cells
DC Field | Value | Language |
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dc.contributor.author | Jung, Ji-Hye | - |
dc.contributor.author | Lee, Seung Jin | - |
dc.contributor.author | Kim, JiHea | - |
dc.contributor.author | Lee, SongHee | - |
dc.contributor.author | Sung, Hwa-Jung | - |
dc.contributor.author | An, Jungsuk | - |
dc.contributor.author | Park, Yong | - |
dc.contributor.author | Kim, Byung Soo | - |
dc.date.accessioned | 2021-09-04T17:06:17Z | - |
dc.date.available | 2021-09-04T17:06:17Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2015-04-15 | - |
dc.identifier.issn | 1547-3287 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/93830 | - |
dc.description.abstract | Basic fibroblast growth factor (bFGF) is a crucial factor sustaining human pluripotent stem cells (hPSCs). We designed this study to search the substitutive factors other than bFGF for the maintenance of hPSCs by using human placenta-derived conditioned medium without exogenous bFGF (hPCCM-), containing chemokine (C-X-C motif) receptor 2 (CXCR2) ligands, including interleukin (IL)-8 and growth-related oncogene alpha (GRO alpha), which were developed on the basis of our previous studies. First, we confirmed that IL-8 and/or GRO alpha play independent roles to preserve the phenotype of hPSCs. Then, we tried CXCR2 blockage of hPSCs in hPCCM- and verified the significant decrease of pluripotency-associated genes expression and the proliferation of hPSCs. Interestingly, CXCR2 suppression of hPSCs in mTeSR (TM) 1 containing exogenous bFGF decreased the proliferation of hPSCs while maintaining pluripotency characteristics. Lastly, we found that hPSCs proliferated robustly for more than 35 passages in hPCCM- on a gelatin substratum. Higher CXCR2 expression of hPSCs cultured in hPCCM- than those in mTeSR (TM) 1 was observable. Our findings suggest that CXCR2 and its related ligands might be novel factors comparable to bFGF supporting the characteristics of hPSCs and hPCCM- might be useful for the maintenance of hPSCs as well as for the accurate evaluation of CXCR2 role in hPSCs without the confounding influence of exogenous bFGF. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MARY ANN LIEBERT, INC | - |
dc.subject | GROWTH-FACTOR SUPPLEMENTATION | - |
dc.subject | HUMAN PLACENTA | - |
dc.subject | UNDIFFERENTIATED PROPAGATION | - |
dc.subject | CONDITIONED MEDIUM | - |
dc.subject | SELF-RENEWAL | - |
dc.subject | FREE CULTURE | - |
dc.subject | FEEDER | - |
dc.subject | MAINTENANCE | - |
dc.subject | SENESCENCE | - |
dc.subject | APOPTOSIS | - |
dc.title | CXCR2 and Its Related Ligands Play a Novel Role in Supporting the Pluripotency and Proliferation of Human Pluripotent Stem Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sung, Hwa-Jung | - |
dc.contributor.affiliatedAuthor | Park, Yong | - |
dc.contributor.affiliatedAuthor | Kim, Byung Soo | - |
dc.identifier.doi | 10.1089/scd.2014.0381 | - |
dc.identifier.scopusid | 2-s2.0-84926451189 | - |
dc.identifier.wosid | 000352322200004 | - |
dc.identifier.bibliographicCitation | STEM CELLS AND DEVELOPMENT, v.24, no.8, pp.948 - 961 | - |
dc.relation.isPartOf | STEM CELLS AND DEVELOPMENT | - |
dc.citation.title | STEM CELLS AND DEVELOPMENT | - |
dc.citation.volume | 24 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 948 | - |
dc.citation.endPage | 961 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Hematology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Transplantation | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Hematology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Transplantation | - |
dc.subject.keywordPlus | GROWTH-FACTOR SUPPLEMENTATION | - |
dc.subject.keywordPlus | HUMAN PLACENTA | - |
dc.subject.keywordPlus | UNDIFFERENTIATED PROPAGATION | - |
dc.subject.keywordPlus | CONDITIONED MEDIUM | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | FREE CULTURE | - |
dc.subject.keywordPlus | FEEDER | - |
dc.subject.keywordPlus | MAINTENANCE | - |
dc.subject.keywordPlus | SENESCENCE | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordAuthor | CXCR2 human pluripotent stem cells | - |
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