CXCR2 and Its Related Ligands Play a Novel Role in Supporting the Pluripotency and Proliferation of Human Pluripotent Stem Cells
- Authors
- Jung, Ji-Hye; Lee, Seung Jin; Kim, JiHea; Lee, SongHee; Sung, Hwa-Jung; An, Jungsuk; Park, Yong; Kim, Byung Soo
- Issue Date
- 15-4월-2015
- Publisher
- MARY ANN LIEBERT, INC
- Keywords
- CXCR2 human pluripotent stem cells
- Citation
- STEM CELLS AND DEVELOPMENT, v.24, no.8, pp.948 - 961
- Indexed
- SCIE
SCOPUS
- Journal Title
- STEM CELLS AND DEVELOPMENT
- Volume
- 24
- Number
- 8
- Start Page
- 948
- End Page
- 961
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/93830
- DOI
- 10.1089/scd.2014.0381
- ISSN
- 1547-3287
- Abstract
- Basic fibroblast growth factor (bFGF) is a crucial factor sustaining human pluripotent stem cells (hPSCs). We designed this study to search the substitutive factors other than bFGF for the maintenance of hPSCs by using human placenta-derived conditioned medium without exogenous bFGF (hPCCM-), containing chemokine (C-X-C motif) receptor 2 (CXCR2) ligands, including interleukin (IL)-8 and growth-related oncogene alpha (GRO alpha), which were developed on the basis of our previous studies. First, we confirmed that IL-8 and/or GRO alpha play independent roles to preserve the phenotype of hPSCs. Then, we tried CXCR2 blockage of hPSCs in hPCCM- and verified the significant decrease of pluripotency-associated genes expression and the proliferation of hPSCs. Interestingly, CXCR2 suppression of hPSCs in mTeSR (TM) 1 containing exogenous bFGF decreased the proliferation of hPSCs while maintaining pluripotency characteristics. Lastly, we found that hPSCs proliferated robustly for more than 35 passages in hPCCM- on a gelatin substratum. Higher CXCR2 expression of hPSCs cultured in hPCCM- than those in mTeSR (TM) 1 was observable. Our findings suggest that CXCR2 and its related ligands might be novel factors comparable to bFGF supporting the characteristics of hPSCs and hPCCM- might be useful for the maintenance of hPSCs as well as for the accurate evaluation of CXCR2 role in hPSCs without the confounding influence of exogenous bFGF.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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