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Transfer of Tetracycline Resistance Genes with Aggregation Substance in Food-Borne Enterococcus faecalis

Authors
Choi, Jong-MiWoo, Gun-Jo
Issue Date
4월-2015
Publisher
SPRINGER
Citation
CURRENT MICROBIOLOGY, v.70, no.4, pp.476 - 484
Indexed
SCIE
SCOPUS
Journal Title
CURRENT MICROBIOLOGY
Volume
70
Number
4
Start Page
476
End Page
484
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/93933
DOI
10.1007/s00284-014-0742-1
ISSN
0343-8651
Abstract
Enterococcus faecalis has the ability to conjugate with the aid of aggregation substance (AS) and inducible sex pheromones to exchange genetic elements in food matrix. To evaluate the food safety condition and the transferable factor, 250 tetracycline-resistant food-borne E. faecalis were collected in Korea. Among the isolates, a majority of tetracycline-resistant isolates (49.6 %) harbored both the tet(M) and tet(L) genes together, followed by tet(M) (19.6 %), and tet(L) (6.8 %) alone. Also, we found the combination of tet(L)/tet(M)/tet(O) or tet(M)/tet(O). We identified two tet(S) genes including the isolate carrying tet(M) + tet(S) genes. Additionally, most E. faecalis were positive for cpd and ccf (both 96.8 %) followed by cob (57.2 %). Through mating experiments, we confirmed E. faecalis possessing the Int-Tn gene and/or any AS gene successfully transferred tet genes to JH2-2 E. faecalis, whereas neither E. faecalis carrying AS genes nor the Int-Tn gene showed the conjugation. Pulsed-field gel electrophoresis results supported a distinct pattern, implying transfer of genetic information. Our study revealed a high occurrence of tetracycline resistance genes in E. faecalis from various foods. The widespread dissemination of tetracycline resistance genes would be promoted to transfer tetracycline resistance genes by pheromone-mediated conjugation systems.
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