6-Gingerol reduces Pseudomonas aeruginosa biofilm formation and virulence via quorum sensing inhibition
- Authors
- Kim, Han-Shin; Lee, Sang-Hoon; Byun, Youngjoo; Park, Hee-Deung
- Issue Date
- 2-3월-2015
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- SCIENTIFIC REPORTS, v.5
- Indexed
- SCIE
SCOPUS
- Journal Title
- SCIENTIFIC REPORTS
- Volume
- 5
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/94160
- DOI
- 10.1038/srep08656
- ISSN
- 2045-2322
- Abstract
- Pseudomonas aeruginosa is a well-known pathogenic bacterium that forms biofilms and produces virulence factors via quorum sensing (QS). Interfering with normal QS interactions between signal molecules and their cognate receptors is a developing strategy for attenuating its virulence. Here we tested the hypothesis that 6-gingerol, a pungent oil of fresh ginger, reduces biofilm formation and virulence by antagonistically binding to P. aeruginosa QS receptors. In silico studies demonstrated molecular binding occurs between 6-gingerol and the QS receptor LasR through hydrogen bonding and hydrophobic interactions. Experimentally 6-gingerol reduced biofilm formation, several virulence factors (e.g., exoprotease, rhamnolipid, and pyocyanin), and mice mortality. Further transcriptome analyses demonstrated that 6-gingerol successfully repressed QS-induced genes, specifically those related to the production of virulence factors. These results strongly support our hypothesis and offer insight into the molecular mechanism that caused QS gene repression.
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Collections - College of Pharmacy > Department of Pharmaceutical Science > 1. Journal Articles
- College of Engineering > School of Civil, Environmental and Architectural Engineering > 1. Journal Articles
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