Collagen-based brain microvasculature model in vitro using three-dimensional printed template
- Authors
- Kim, Jeong Ah; Kim, Hong Nam; Im, Sun-Kyoung; Chung, Seok; Kang, Ji Yoon; Choi, Nakwon
- Issue Date
- 3월-2015
- Publisher
- AMER INST PHYSICS
- Citation
- BIOMICROFLUIDICS, v.9, no.2
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOMICROFLUIDICS
- Volume
- 9
- Number
- 2
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/94353
- DOI
- 10.1063/1.4917508
- ISSN
- 1932-1058
- Abstract
- We present an engineered three-dimensional (3D) in vitro brain microvasculature system embedded within the bulk of a collagen matrix. To create a hydrogel template for the functional brain microvascular structure, we fabricated an array of microchannels made of collagen I using microneedles and a 3D printed frame. By culturing mouse brain endothelial cells (bEnd.3) on the luminal surface of cylindrical collagen microchannels, we reconstructed an array of brain microvasculature in vitro with circular cross-sections. We characterized the barrier function of our brain microvasculature by measuring transendothelial permeability of 40 kDa fluorescein isothiocyanate-dextran (Stoke's radius of similar to 4.5 nm), based on an analytical model. The transendothelial permeability decreased significantly over 3 weeks of culture. We also present the disruption of the barrier function with a hyperosmotic mannitol as well as a subsequent recovery over 4 days. Our brain microvasculature model in vitro, consisting of system-in-hydrogel combined with the widely emerging 3D printing technique, can serve as a useful tool not only for fundamental studies associated with blood-brain barrier in physiological and pathological settings but also for pharmaceutical applications. (C) 2015 AIP Publishing LLC.
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Collections - College of Engineering > Department of Mechanical Engineering > 1. Journal Articles
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