Simultaneous analysis of 210 prohibited substances in human urine by ultrafast liquid chromatography/tandem mass spectrometry in doping control
- Authors
- Jeong, Eun Sook; Kim, So-Hee; Cha, Eun-Ju; Lee, Kang Mi; Kim, Ho Jun; Lee, Sang-Won; Kwon, Oh-Seung; Lee, Jaeick
- Issue Date
- 28-2월-2015
- Publisher
- WILEY
- Citation
- RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v.29, no.4, pp.367 - 384
- Indexed
- SCIE
SCOPUS
- Journal Title
- RAPID COMMUNICATIONS IN MASS SPECTROMETRY
- Volume
- 29
- Number
- 4
- Start Page
- 367
- End Page
- 384
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/94369
- DOI
- 10.1002/rcm.7113
- ISSN
- 0951-4198
- Abstract
- RATIONALE: Doping analysis is a two-step process consisting of a screening step for prohibited substances and a confirmation step to verify the presence of specific substances found during the screening. The entire process must be performed within a limited time period, but traditional screening procedures commonly employ separate analytical methods for each class of prohibited substances being screened and thus require a great deal of human resources and instrumentation. A single simple and rapid multiresidue analytical method that could accommodate multiple classes of prohibited substances would be extraordinarily useful in doping analyses. METHODS: Urine samples were extracted via two consecutive liquid-liquid extractions at different pH values following enzymatic hydrolysis. Analyses were performed by ultrafast liquid chromatography/triple-quadrupole mass spectrometry with polarity switching and time-dependent selected reaction monitoring. RESULTS: We developed a rapid multiresidue screening and confirmation method for efficient high-throughput doping analyses. The present method was validated with regard to the limits of detection (0.01-100.0 ng/mL for screening analyses and 0.2-500.0 ng/mL for confirmation assays), matrix effects (48.9-118.9%), recovery (20.6-119.7%) and intra( 0.6-17.6%) and inter-day (4.0-20.0%) precision. CONCLUSIONS: A multiresidue analytical method was developed and validated for screening and confirming the presence of performance-enhancing drugs. A total of 210 substances from diverse classes of prohibited substances were successfully identified with an analytical run time of 10 min. Copyright (C) 2015 John Wiley & Sons, Ltd.
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