Antifibrotic Compounds from Liriodendron tulipifera Attenuating HSC-T6 Proliferation and TNF-alpha Production in RAW264.7 Cells
- Authors
- Jeong, Eun Ju; Kim, Na-Hyun; Heo, Jeong-Doo; Lee, Ki Yong; Rho, Jung-Rae; Kim, Young Choong; Sung, Sang Hyun
- Issue Date
- Feb-2015
- Publisher
- PHARMACEUTICAL SOC JAPAN
- Keywords
- Liriodendron tulipifera; antifibrotic; hepatic stellate cell; HSC-T6; macrophage; RAW264.7
- Citation
- BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.38, no.2, pp.228 - 234
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOLOGICAL & PHARMACEUTICAL BULLETIN
- Volume
- 38
- Number
- 2
- Start Page
- 228
- End Page
- 234
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/94501
- DOI
- 10.1248/bpb.b14-00583
- ISSN
- 0918-6158
- Abstract
- The inhibition of hepatic stellate cell (HSC) proliferation has been considered as an effective therapeutic target for the treatment of liver fibrosis. The methanolic extract of Liriodendron tulipifera showed significant inhibitory activity against the proliferation of HSCs. Bioactivity-guided isolation afforded twelve compounds including (-)-sesamin (1), (-)-syringaresinol (2), (+)-dihydrodehydrodiconiferyl alcohol (3), salvinal (4), (+)-guaiacylglycerol-8-O-4'-dihydroconiferyl ether (5), (+/-)-guaiacylglycerol-8-O-4'-sinapyl alcohol ether (6), tanegool (7), (+)-5,5'-dimethoxy-7-oxolariciresinol (8), 3-hydroxy-4-methoxyacetophenone (9), 4-acetoxymethylphenol (10), (-)-paramicholide (11), and blumenol A (12). Among the compounds isolated, 2, 3 and 4 significantly attenuated the proliferation of the activated HSC-T6 cells. The maximal dose of these compounds, however, showed no cytotoxicity in primary cultured rat hepatocytes. Collagen deposition in the activated HSC-T6 cells was reduced by 2, 3 and 4. Also, the increased production of the pro-inflammatory cytokine tumor necrosis factor (TNF)-alpha induced by lipopolysaccharide was decreased by 3 and 4 in RAW264.7 macrophage cells. Collectively, (-)-syringaresinol (2), (+)-dihydrodehydrodiconiferyl alcohol (3), and salvinal (4) isolated from L. tulipifera leaves and twigs exhibited selective antifibrotic activities toward the activated HSCs and suppressed TNF-alpha production in RAW264.7 macrophages. These compounds may be useful candidates for developing therapeutic agents for the prevention and treatment of hepatic fibrosis.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Pharmacy > Department of Pharmaceutical Science > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholar.korea.ac.kr/handle/2021.sw.korea/94501)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.