Human amniotic membrane-derived stromal cells (hAMSC) interact depending on breast cancer cell type through secreted molecules
- Authors
- Kim, Sun-Hee; Bang, So Hee; Kang, So Yeong; Park, Ki Dae; Eom, Jun Ho; Oh, Il Ung; Yoo, Si Hyung; Kim, Chan-Wha; Baek, Sun Young
- Issue Date
- 2월-2015
- Publisher
- CHURCHILL LIVINGSTONE
- Keywords
- Amniotic membrane; MCF-7; MDA-MB-231; Secretion
- Citation
- TISSUE & CELL, v.47, no.1, pp.10 - 16
- Indexed
- SCIE
SCOPUS
- Journal Title
- TISSUE & CELL
- Volume
- 47
- Number
- 1
- Start Page
- 10
- End Page
- 16
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/94583
- DOI
- 10.1016/j.tice.2014.10.003
- ISSN
- 0040-8166
- Abstract
- Human amniotic membrane-derived stromal cells (hAMSC) are candidates for cell-based therapies. We examined the characteristics of hAMSC including the interaction between hAMSC and breast cancer cells, MCF-7, and MDA-MB-231. Human amniotic membrane-derived stromal cells showed typical MSC properties, including fibroblast-like morphology, surface antigen expression, and mesodermal differentiation. To investigate cell-cell interaction via secreted molecules, we cultured breast cancer cells in hAMSC-conditioned medium (hAMSC-CM) and analyzed their proliferation, migration, and secretome profiles. MCF-7 and MDA-MB-231 cells exposed to hAMSC-CM showed increased proliferation and migration. However, in hAMSC-CM, MCF-7 cells proliferated significantly faster than MDA-MB-231 cells. When cultured in hAMSC-CM, MCF-7 cells migrated faster than MDA-MB-231 cells. Two cell types showed different profiles of secreted factors. MCF-7 cells expressed much amounts of IL-8, GRO, and MCP-1 in hAMSC-CM. Human amniotic membrane-derived stromal cells interact with breast cancer cells through secreted molecules. Factors secreted by hAMSCs promote the proliferation and migration of MCF-7 breast cancer cells. For much safe cell-based therapies using hAMSC, it is necessary to study carefully about interaction between hAMSC and cancer cells. (C) 2014 Elsevier Ltd. All rights reserved.
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Collections - College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
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