Evaluation and Verification of the Nanosphere Verigene RV plus Assay for Detection of Influenza A/B and H1/H3 Subtyping
DC Field | Value | Language |
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dc.contributor.author | Cho, Han Jin | - |
dc.contributor.author | Jang, Jin Woo | - |
dc.contributor.author | Ko, Sun Young | - |
dc.contributor.author | Choi, Sung Hyuk | - |
dc.contributor.author | Lim, Chae Seung | - |
dc.contributor.author | An, Seong Soo A. | - |
dc.date.accessioned | 2021-09-04T20:29:27Z | - |
dc.date.available | 2021-09-04T20:29:27Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2015-01 | - |
dc.identifier.issn | 0146-6615 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/94782 | - |
dc.description.abstract | With the emerging risks of drug-resistant viruses and pandemic influenza, rapid and accurate detection of influenza viruses and determination of their subtypes is a crucial component of patient management. This study evaluated the performance of the Verigene respiratory virus plus nucleic acid (Verigene RV+) test for the detection of influenza A/B and subtype determination compared it with conventional molecular methods. Nasopharyngeal swabs were collected from 228 patients with influenza-like illness (influenza A (n=67), 2009-H1N1 (n=21), influenza B (n=80), mixed A & B (n=3), mixed RSV A and influenza (n=3), and influenza-negative (n=54)). Patient samples were analyzed by Influenza A/B one-step typing (Seegene, Seoul, Korea), Seeplex RV15 ACE Detection (Seegene), Nanosphere Verigene RV+ assay (Nanosphere, Northbrrook, IL) and virus culture. Out of 228 samples, 109 (47.8%) were positive by culture, and an additional 65 (28.5%) were positive by Seeplex RV15 ACE Detection, Influenza A/B one-step typing or Nanosphere Verigene RV+ assay. In comparison tests with Seeplex RV15 ACE Detection RT-PCR, the sensitivity of the Verigene RV+ kit for detection of the influenza A, 2009-H1N1, influenza B, and mixed A & B was 97.1%, 100%, 100%, and 100%, respectively. The specificity of the Verigene RV+ was 100% for all types. The concordance between Verigene RV+ and Influenza A/B one-step typing for H1, H3, H1/H3 mixed, and 2009-H1N1 was 100% (26/26), 100% (35/35), 100% (4/4), and 100% (21/21), respectively. The Verigene RV+ assay showed acceptable sensitivity and specificity for detection and subtyping of influenza viruses compared with the conventional RT-PCR method. J. Med. Virol. 87: 18-24, 2015. (c) 2014 Wiley Periodicals, Inc. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | REAL-TIME PCR | - |
dc.subject | A H1N1 VIRUS | - |
dc.subject | RESPIRATORY VIRUSES | - |
dc.subject | XPERT FLU | - |
dc.subject | RT-PCR | - |
dc.subject | AMPLIFICATION | - |
dc.subject | PANEL | - |
dc.subject | PERFORMANCE | - |
dc.subject | PATHOGENS | - |
dc.subject | DIAGNOSIS | - |
dc.title | Evaluation and Verification of the Nanosphere Verigene RV plus Assay for Detection of Influenza A/B and H1/H3 Subtyping | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Cho, Han Jin | - |
dc.contributor.affiliatedAuthor | Ko, Sun Young | - |
dc.contributor.affiliatedAuthor | Choi, Sung Hyuk | - |
dc.contributor.affiliatedAuthor | Lim, Chae Seung | - |
dc.identifier.doi | 10.1002/jmv.23970 | - |
dc.identifier.scopusid | 2-s2.0-84909601810 | - |
dc.identifier.wosid | 000344802800003 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MEDICAL VIROLOGY, v.87, no.1, pp.18 - 24 | - |
dc.relation.isPartOf | JOURNAL OF MEDICAL VIROLOGY | - |
dc.citation.title | JOURNAL OF MEDICAL VIROLOGY | - |
dc.citation.volume | 87 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 18 | - |
dc.citation.endPage | 24 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Virology | - |
dc.relation.journalWebOfScienceCategory | Virology | - |
dc.subject.keywordPlus | REAL-TIME PCR | - |
dc.subject.keywordPlus | A H1N1 VIRUS | - |
dc.subject.keywordPlus | RESPIRATORY VIRUSES | - |
dc.subject.keywordPlus | XPERT FLU | - |
dc.subject.keywordPlus | RT-PCR | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | PANEL | - |
dc.subject.keywordPlus | PERFORMANCE | - |
dc.subject.keywordPlus | PATHOGENS | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordAuthor | molecular | - |
dc.subject.keywordAuthor | diagnostic | - |
dc.subject.keywordAuthor | influenza | - |
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