Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Evaluation and Verification of the Nanosphere Verigene RV plus Assay for Detection of Influenza A/B and H1/H3 Subtyping

Authors
Cho, Han JinJang, Jin WooKo, Sun YoungChoi, Sung HyukLim, Chae SeungAn, Seong Soo A.
Issue Date
1월-2015
Publisher
WILEY
Keywords
molecular; diagnostic; influenza
Citation
JOURNAL OF MEDICAL VIROLOGY, v.87, no.1, pp.18 - 24
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF MEDICAL VIROLOGY
Volume
87
Number
1
Start Page
18
End Page
24
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/94782
DOI
10.1002/jmv.23970
ISSN
0146-6615
Abstract
With the emerging risks of drug-resistant viruses and pandemic influenza, rapid and accurate detection of influenza viruses and determination of their subtypes is a crucial component of patient management. This study evaluated the performance of the Verigene respiratory virus plus nucleic acid (Verigene RV+) test for the detection of influenza A/B and subtype determination compared it with conventional molecular methods. Nasopharyngeal swabs were collected from 228 patients with influenza-like illness (influenza A (n=67), 2009-H1N1 (n=21), influenza B (n=80), mixed A & B (n=3), mixed RSV A and influenza (n=3), and influenza-negative (n=54)). Patient samples were analyzed by Influenza A/B one-step typing (Seegene, Seoul, Korea), Seeplex RV15 ACE Detection (Seegene), Nanosphere Verigene RV+ assay (Nanosphere, Northbrrook, IL) and virus culture. Out of 228 samples, 109 (47.8%) were positive by culture, and an additional 65 (28.5%) were positive by Seeplex RV15 ACE Detection, Influenza A/B one-step typing or Nanosphere Verigene RV+ assay. In comparison tests with Seeplex RV15 ACE Detection RT-PCR, the sensitivity of the Verigene RV+ kit for detection of the influenza A, 2009-H1N1, influenza B, and mixed A & B was 97.1%, 100%, 100%, and 100%, respectively. The specificity of the Verigene RV+ was 100% for all types. The concordance between Verigene RV+ and Influenza A/B one-step typing for H1, H3, H1/H3 mixed, and 2009-H1N1 was 100% (26/26), 100% (35/35), 100% (4/4), and 100% (21/21), respectively. The Verigene RV+ assay showed acceptable sensitivity and specificity for detection and subtyping of influenza viruses compared with the conventional RT-PCR method. J. Med. Virol. 87: 18-24, 2015. (c) 2014 Wiley Periodicals, Inc.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Medicine > Department of Medical Science > 1. Journal Articles
Graduate School > Department of Biomedical Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Choi, Sung Hyuk photo

Choi, Sung Hyuk
의과대학 (의학과)
Read more

Altmetrics

Total Views & Downloads

BROWSE