GLYCINE-RICH RNA-BINDING PROTEIN1 interacts with RECEPTOR-LIKE CYTOPLASMIC PROTEIN KINASE1 and suppresses cell death and defense responses in pepper (Capsicum annuum)
DC Field | Value | Language |
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dc.contributor.author | Kim, Dae Sung | - |
dc.contributor.author | Kim, Nak Hyun | - |
dc.contributor.author | Hwang, Byung Kook | - |
dc.date.accessioned | 2021-09-04T20:41:27Z | - |
dc.date.available | 2021-09-04T20:41:27Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2015-01 | - |
dc.identifier.issn | 0028-646X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/94862 | - |
dc.description.abstract | Plants use a variety of innate immune regulators to trigger cell death and defense responses against pathogen attack. We identified pepper (Capsicum annuum) GLYCINE-RICH RNA-BINDING PROTEIN1 (CaGRP1) as a RECEPTOR-LIKE CYTOPLASMIC PROTEIN KINASE1 (CaPIK1)-interacting partner, based on bimolecular fluorescence complementation and coimmunoprecipitation analyses as well as gene silencing and transient expression analysis. CaGRP1 contains an N-terminal RNA recognition motif and a glycine-rich region at the C-terminus. The CaGRP1 protein had DNA- and RNA-binding activity in vitro. CaGRP1 interacted with CaPIK1 in planta. CaGRP1 and CaGRP1-CaPIK1 complexes were localized to the nucleus in plant cells. CaPIK1 phosphorylated CaGRP1 in vitro and in planta. Transient coexpression of CaGRP1 with CaPIK1 suppressed the CaPIK1-triggered cell death response, accompanied by a reduced CaPIK1-triggered reactive oxygen species (ROS) burst. The RNA recognition motif region of CaGRP1 was responsible for the nuclear localization of CaGRP1 as well as the suppression of the CaPIK1-triggered cell death response. CaGRP1 silencing in pepper conferred enhanced resistance to Xanthomonas campestris pv vesicatoria (Xcv) infection; however, CaPIK1-silenced plants were more susceptible to Xcv. CaGRP1 interacts with CaPIK1 and negatively regulates CaPIK1-triggered cell death and defense responses by suppressing ROS accumulation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | MESSENGER-RNA | - |
dc.subject | ARABIDOPSIS-THALIANA | - |
dc.subject | RECOGNITION MOTIF | - |
dc.subject | NUCLEAR-RNA | - |
dc.subject | GENE | - |
dc.subject | EXPRESSION | - |
dc.subject | PATHOGEN | - |
dc.subject | SEQUENCE | - |
dc.subject | DISEASE | - |
dc.subject | INFECTION | - |
dc.title | GLYCINE-RICH RNA-BINDING PROTEIN1 interacts with RECEPTOR-LIKE CYTOPLASMIC PROTEIN KINASE1 and suppresses cell death and defense responses in pepper (Capsicum annuum) | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hwang, Byung Kook | - |
dc.identifier.doi | 10.1111/nph.13105 | - |
dc.identifier.scopusid | 2-s2.0-84919608714 | - |
dc.identifier.wosid | 000346584600033 | - |
dc.identifier.bibliographicCitation | NEW PHYTOLOGIST, v.205, no.2, pp.786 - 800 | - |
dc.relation.isPartOf | NEW PHYTOLOGIST | - |
dc.citation.title | NEW PHYTOLOGIST | - |
dc.citation.volume | 205 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 786 | - |
dc.citation.endPage | 800 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | ARABIDOPSIS-THALIANA | - |
dc.subject.keywordPlus | RECOGNITION MOTIF | - |
dc.subject.keywordPlus | NUCLEAR-RNA | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PATHOGEN | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordAuthor | cell death | - |
dc.subject.keywordAuthor | DNA and RNA binding | - |
dc.subject.keywordAuthor | glycine-rich RNA-binding protein | - |
dc.subject.keywordAuthor | pepper (Capsicum annuum) | - |
dc.subject.keywordAuthor | plant defense | - |
dc.subject.keywordAuthor | receptor-like cytoplasmic protein kinase | - |
dc.subject.keywordAuthor | Xanthomonas campestris pv vesicatoria (Xcv) | - |
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