GLYCINE-RICH RNA-BINDING PROTEIN1 interacts with RECEPTOR-LIKE CYTOPLASMIC PROTEIN KINASE1 and suppresses cell death and defense responses in pepper (Capsicum annuum)
- Authors
- Kim, Dae Sung; Kim, Nak Hyun; Hwang, Byung Kook
- Issue Date
- 1월-2015
- Publisher
- WILEY
- Keywords
- cell death; DNA and RNA binding; glycine-rich RNA-binding protein; pepper (Capsicum annuum); plant defense; receptor-like cytoplasmic protein kinase; Xanthomonas campestris pv vesicatoria (Xcv)
- Citation
- NEW PHYTOLOGIST, v.205, no.2, pp.786 - 800
- Indexed
- SCIE
SCOPUS
- Journal Title
- NEW PHYTOLOGIST
- Volume
- 205
- Number
- 2
- Start Page
- 786
- End Page
- 800
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/94862
- DOI
- 10.1111/nph.13105
- ISSN
- 0028-646X
- Abstract
- Plants use a variety of innate immune regulators to trigger cell death and defense responses against pathogen attack. We identified pepper (Capsicum annuum) GLYCINE-RICH RNA-BINDING PROTEIN1 (CaGRP1) as a RECEPTOR-LIKE CYTOPLASMIC PROTEIN KINASE1 (CaPIK1)-interacting partner, based on bimolecular fluorescence complementation and coimmunoprecipitation analyses as well as gene silencing and transient expression analysis. CaGRP1 contains an N-terminal RNA recognition motif and a glycine-rich region at the C-terminus. The CaGRP1 protein had DNA- and RNA-binding activity in vitro. CaGRP1 interacted with CaPIK1 in planta. CaGRP1 and CaGRP1-CaPIK1 complexes were localized to the nucleus in plant cells. CaPIK1 phosphorylated CaGRP1 in vitro and in planta. Transient coexpression of CaGRP1 with CaPIK1 suppressed the CaPIK1-triggered cell death response, accompanied by a reduced CaPIK1-triggered reactive oxygen species (ROS) burst. The RNA recognition motif region of CaGRP1 was responsible for the nuclear localization of CaGRP1 as well as the suppression of the CaPIK1-triggered cell death response. CaGRP1 silencing in pepper conferred enhanced resistance to Xanthomonas campestris pv vesicatoria (Xcv) infection; however, CaPIK1-silenced plants were more susceptible to Xcv. CaGRP1 interacts with CaPIK1 and negatively regulates CaPIK1-triggered cell death and defense responses by suppressing ROS accumulation.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Life Sciences and Biotechnology > Division of Biotechnology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.