RAPD분석을 통한 여름양송이(Agaricus bitorquis)의 교잡주 선발

Abstract

Agaricus bitorquis is an edible white mushroom of the genus that is cultivated at high temperature(25±1oC) unlike A. bisporus is cultivate at 16±2oC. Unlike Agaricus bisporus, an edible white A. bitorquis mushroom is cultivated at high temperature (25±1oC). Most farmers cultivate this mushroom for a long cultivation period in Korea. For this reason, we made heterokayons to develop a new cultivar that generate fruitbodies for short cultivation period. Over one hundred SSIs(single spores isolates) were collected from selected A. bitorquis ASI1151 and ASI1349 strains. Seventy-three SSIs were germinated on CDA(compost dextrose agar) media after 20 days (minimum) or 83 days (maximum) incubation under different media condition. The mycelial growth rate of germinated SSIs was different. 9 homokaryons in ASI 1151 and 11 homokaryons in ASI 1349 from SSIs were selected by OPN-02 primer in RAPD analaysis. Also this primer was used to select heterokaryon that cross among each homokaryon with compatible locus. Therefore 44 compatible matings were confirmed of 99 crossed lines.