휴경지 토양에서 수거된 파리 번데기의 분포 및 냉해를 입은 구더기를 이용한 사후 경과시간 추정 사례

Abstract

Forensic entomology investigates postmortem interval (PMI) estimation using insectevidence. We estimated the minimum PMI of a putrefied male cadaver using pupae inthe soil and maggots found in the body. Most of the maggots, collected during theautopsy, were postfeeding third instar larvae with empty crop contents, which developedfreezing injuries when the cadaver was placed in the freezer. Pupae in the soilwere collected 45 days after the discovery of the body. DNA barcoding revealed thatmost pupae and maggots were Chrysomya pinguis, with a few exceptions. The minimumPMI was estimated at 10 days before the discovery time based on the sceneinvestigation, maggot developmental stage, distribution of pupae moving away fromthe body toward pupariation sites, DNA barcoding results, and weather information. To reduce the gap between the minimum and maximum PMI values, complete entomologicalevidence collection should be conducted at the time of discovery.